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| | ==Solution NMR structure of the Mus81 N-terminal HhH. Northeast Structural Genomics Consortium target MmT1A== | | ==Solution NMR structure of the Mus81 N-terminal HhH. Northeast Structural Genomics Consortium target MmT1A== |
| - | <StructureSection load='2kp7' size='340' side='right'caption='[[2kp7]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2kp7' size='340' side='right'caption='[[2kp7]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kp7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KP7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kp7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KP7 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mus81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kp7 OCA], [http://pdbe.org/2kp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kp7 RCSB], [http://www.ebi.ac.uk/pdbsum/2kp7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kp7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kp7 OCA], [https://pdbe.org/2kp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kp7 RCSB], [https://www.ebi.ac.uk/pdbsum/2kp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kp7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MUS81_MOUSE MUS81_MOUSE]] Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:14609959</ref> <ref>PMID:15205536</ref> <ref>PMID:16107704</ref> | + | [https://www.uniprot.org/uniprot/MUS81_MOUSE MUS81_MOUSE] Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:14609959</ref> <ref>PMID:15205536</ref> <ref>PMID:16107704</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Arrowsmith, C H]] | + | [[Category: Arrowsmith CH]] |
| - | [[Category: Laister, R C]] | + | [[Category: Laister RC]] |
| - | [[Category: Lemak, A]] | + | [[Category: Lemak A]] |
| - | [[Category: Montelione, G T]] | + | [[Category: Montelione GT]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Wu B]] |
| - | [[Category: Wu, B]] | + | |
| - | [[Category: Dna damage]]
| + | |
| - | [[Category: Dna recombination]]
| + | |
| - | [[Category: Dna repair]]
| + | |
| - | [[Category: Endonuclease]]
| + | |
| - | [[Category: Helix-hairpin-helix]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Magnesium]]
| + | |
| - | [[Category: Metal-binding]]
| + | |
| - | [[Category: Nesg]]
| + | |
| - | [[Category: Nuclease]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Tumour suppressor]]
| + | |
| Structural highlights
Function
MUS81_MOUSE Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Abraham J, Lemmers B, Hande MP, Moynahan ME, Chahwan C, Ciccia A, Essers J, Hanada K, Chahwan R, Khaw AK, McPherson P, Shehabeldin A, Laister R, Arrowsmith C, Kanaar R, West SC, Jasin M, Hakem R. Eme1 is involved in DNA damage processing and maintenance of genomic stability in mammalian cells. EMBO J. 2003 Nov 17;22(22):6137-47. PMID:14609959 doi:http://dx.doi.org/10.1093/emboj/cdg580
- ↑ McPherson JP, Lemmers B, Chahwan R, Pamidi A, Migon E, Matysiak-Zablocki E, Moynahan ME, Essers J, Hanada K, Poonepalli A, Sanchez-Sweatman O, Khokha R, Kanaar R, Jasin M, Hande MP, Hakem R. Involvement of mammalian Mus81 in genome integrity and tumor suppression. Science. 2004 Jun 18;304(5678):1822-6. PMID:15205536 doi:http://dx.doi.org/10.1126/science.1094557
- ↑ Dendouga N, Gao H, Moechars D, Janicot M, Vialard J, McGowan CH. Disruption of murine Mus81 increases genomic instability and DNA damage sensitivity but does not promote tumorigenesis. Mol Cell Biol. 2005 Sep;25(17):7569-79. PMID:16107704 doi:http://dx.doi.org/10.1128/MCB.25.17.7569-7579.2005
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