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| | ==Backbone 1H, 13C, and 15N Chemical Shift Assignments for Oryctin== | | ==Backbone 1H, 13C, and 15N Chemical Shift Assignments for Oryctin== |
| - | <StructureSection load='2ksw' size='340' side='right'caption='[[2ksw]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2ksw' size='340' side='right'caption='[[2ksw]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ksw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Coconut_rhinoceros_beetle Coconut rhinoceros beetle]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KSW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ksw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctes_rhinoceros Oryctes rhinoceros]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KSW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KSW FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ksw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksw OCA], [http://pdbe.org/2ksw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ksw RCSB], [http://www.ebi.ac.uk/pdbsum/2ksw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ksw ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ksw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ksw OCA], [https://pdbe.org/2ksw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ksw RCSB], [https://www.ebi.ac.uk/pdbsum/2ksw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ksw ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O96050_ORYRH O96050_ORYRH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Coconut rhinoceros beetle]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Horita, S]] | + | [[Category: Oryctes rhinoceros]] |
| - | [[Category: Ishibashi, J]] | + | [[Category: Horita S]] |
| - | [[Category: Miyakawa, T]] | + | [[Category: Ishibashi J]] |
| - | [[Category: Nagata, K]] | + | [[Category: Miyakawa T]] |
| - | [[Category: Tanokura, M]] | + | [[Category: Nagata K]] |
| - | [[Category: Yamakawa, M]] | + | [[Category: Tanokura M]] |
| - | [[Category: Hydrolase inhibitor]]
| + | [[Category: Yamakawa M]] |
| - | [[Category: Kazal-type]]
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| - | [[Category: Protease inhibitor]]
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| Structural highlights
Function
O96050_ORYRH
Publication Abstract from PubMed
We isolated oryctin, a 66-residue peptide, from the hemolymph of the coconut rhinoceros beetle Oryctes rhinoceros and cloned its cDNA. Oryctin is dissimilar to any other known peptides in amino acid sequence, and its function has been unknown. To reveal that function, we determined the solution structure of recombinant (13)C,(15)N-labeled oryctin by heteronuclear NMR spectroscopy. Oryctin exhibits a fold similar to that of Kazal-type serine protease inhibitors but has a unique additional C-terminal alpha-helix. We performed protease inhibition assays of oryctin against several bacterial and eukaryotic proteases. Oryctin does inhibit the following serine proteases: alpha-chymotrypsin, endopeptidase K, subtilisin Carlsberg, and leukocyte elastase, with K(i) values of 3.9 x 10(-10) m, 6.2 x 10(-10) m, 1.4 x 10(-9) m, and 1.2 x 10(-8) m, respectively. Although the target molecule of oryctin in the beetle hemolymph remains obscure, our results showed that oryctin is a novel single domain Kazal-type inhibitor and could play a key role in protecting against bacterial infections.
Isolation, cDNA cloning, and structure-based functional characterization of oryctin, a hemolymph protein from the coconut rhinoceros beetle, Oryctes rhinoceros, as a novel serine protease inhibitor.,Horita S, Ishibashi J, Nagata K, Miyakawa T, Yamakawa M, Tanokura M J Biol Chem. 2010 Sep 24;285(39):30150-8. Epub 2010 Jul 14. PMID:20630859[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Horita S, Ishibashi J, Nagata K, Miyakawa T, Yamakawa M, Tanokura M. Isolation, cDNA cloning, and structure-based functional characterization of oryctin, a hemolymph protein from the coconut rhinoceros beetle, Oryctes rhinoceros, as a novel serine protease inhibitor. J Biol Chem. 2010 Sep 24;285(39):30150-8. Epub 2010 Jul 14. PMID:20630859 doi:10.1074/jbc.M110.124735
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