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| | <StructureSection load='2c8a' size='340' side='right'caption='[[2c8a]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='2c8a' size='340' side='right'caption='[[2c8a]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2c8a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C8A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2c8a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C8A FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1g24|1g24]], [[1gze|1gze]], [[1gzf|1gzf]], [[1uzi|1uzi]], [[2a78|2a78]], [[2a9k|2a9k]], [[2bov|2bov]], [[2c89|2c89]], [[2c8b|2c8b]], [[2c8c|2c8c]], [[2c8d|2c8d]], [[2c8e|2c8e]], [[2c8f|2c8f]], [[2c8g|2c8g]], [[2c8h|2c8h]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8a OCA], [http://pdbe.org/2c8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c8a RCSB], [http://www.ebi.ac.uk/pdbsum/2c8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2c8a ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c8a OCA], [https://pdbe.org/2c8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c8a RCSB], [https://www.ebi.ac.uk/pdbsum/2c8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c8a ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ARC3_CBDP ARC3_CBDP]] ADP-ribosylates eukaryotic Rho and Rac proteins on an asparagine residue. | + | [https://www.uniprot.org/uniprot/Q7M0L1_CLOBO Q7M0L1_CLOBO] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus botulinus van ermengem 1896]] | + | [[Category: Clostridium botulinum]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boquet, P]] | + | [[Category: Boquet P]] |
| - | [[Category: Flatau, G]] | + | [[Category: Flatau G]] |
| - | [[Category: Menetrey, J]] | + | [[Category: Menetrey J]] |
| - | [[Category: Menez, A]] | + | [[Category: Menez A]] |
| - | [[Category: Stura, E A]] | + | [[Category: Stura EA]] |
| - | [[Category: Artt motif]]
| + | |
| - | [[Category: Bacterial toxin]]
| + | |
| - | [[Category: C3 exoenzyme]]
| + | |
| - | [[Category: Glycosyltransferase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q7M0L1_CLOBO
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
C3-like exoenzymes are ADP-ribosyltransferases that specifically modify some Rho GTPase proteins, leading to their sequestration in the cytoplasm, and thus inhibiting their regulatory activity on the actin cytoskeleton. This modification process goes through three sequential steps involving NAD-hydrolysis, Rho recognition, and binding, leading to Rho ADP-ribosylation. Independently, three distinct residues within the ARTT loop of the C3 exoenzymes are critical for each of these steps. Supporting the critical role of the ARTT loop, we have shown previously that it adopts a distinct conformation upon NAD binding. Here, we present seven wild-type and ARTT loop-mutant structures of C3 exoenzyme of Clostridium botulinum free and bound to its true substrate, NAD, and to its NAD-hydrolysis product, nicotinamide. Altogether, these structures expand our understanding of the conformational diversity of the C3 exoenzyme, mainly within the ARTT loop.
Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes.,Menetrey J, Flatau G, Boquet P, Menez A, Stura EA Protein Sci. 2008 May;17(5):878-86. Epub 2008 Mar 27. PMID:18369192[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Menetrey J, Flatau G, Boquet P, Menez A, Stura EA. Structural basis for the NAD-hydrolysis mechanism and the ARTT-loop plasticity of C3 exoenzymes. Protein Sci. 2008 May;17(5):878-86. Epub 2008 Mar 27. PMID:18369192 doi:10.1110/ps.073398508
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