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| | ==Solution Structure of the Human Immunodeficiency Virus Type 1 p6 Protein== | | ==Solution Structure of the Human Immunodeficiency Virus Type 1 p6 Protein== |
| - | <StructureSection load='2c55' size='340' side='right'caption='[[2c55]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='2c55' size='340' side='right'caption='[[2c55]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2c55]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C55 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C55 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2c55]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C55 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C55 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c55 OCA], [http://pdbe.org/2c55 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2c55 RCSB], [http://www.ebi.ac.uk/pdbsum/2c55 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2c55 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c55 OCA], [https://pdbe.org/2c55 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c55 RCSB], [https://www.ebi.ac.uk/pdbsum/2c55 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c55 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GAG_HV1N5 GAG_HV1N5]] Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity). | + | [https://www.uniprot.org/uniprot/GAG_HV1N5 GAG_HV1N5] Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity). |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Human immunodeficiency virus 1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bruns, K]] | + | [[Category: Bruns K]] |
| - | [[Category: Fossen, T]] | + | [[Category: Fossen T]] |
| - | [[Category: Henklein, P]] | + | [[Category: Henklein P]] |
| - | [[Category: Klinger, P]] | + | [[Category: Klinger P]] |
| - | [[Category: Maczurek, A]] | + | [[Category: Maczurek A]] |
| - | [[Category: Rachmat, J]] | + | [[Category: Rachmat J]] |
| - | [[Category: Schubert, U]] | + | [[Category: Schubert U]] |
| - | [[Category: Tessmer, U]] | + | [[Category: Tessmer U]] |
| - | [[Category: Wray, V]] | + | [[Category: Wray V]] |
| - | [[Category: Aid]]
| + | |
| - | [[Category: Core protein]]
| + | |
| - | [[Category: Hiv-1]]
| + | |
| - | [[Category: Lipoprotein]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Metal-binding]]
| + | |
| - | [[Category: Myristate]]
| + | |
| - | [[Category: P6]]
| + | |
| - | [[Category: P6-gag]]
| + | |
| - | [[Category: Phosphorylation]]
| + | |
| - | [[Category: Polyprotein]]
| + | |
| - | [[Category: Rna-binding]]
| + | |
| - | [[Category: Viral nucleoprotein]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| - | [[Category: Zinc]]
| + | |
| - | [[Category: Zinc-finger]]
| + | |
| Structural highlights
Function
GAG_HV1N5 Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The human immunodeficiency virus type 1 p6 protein represents a docking site for several cellular and viral binding factors and fulfills major roles in the formation of infectious viruses. To date, however, the structure of this 52-amino acid protein, by far the smallest lentiviral protein known, either in its mature form as free p6 or as the C-terminal part of the Pr55 Gag polyprotein has not been unraveled. We have explored the high resolution structure and folding of p6 by CD and NMR spectroscopy. Under membranous solution conditions, p6 can adopt a helix-flexible helix structure; a short helix-1 (amino acids 14-18) is connected to a pronounced helix-2 (amino acids 33-44) by a flexible hinge region. Thus, p6 can be subdivided into two distinct structural and functional domains; helix-2 perfectly defines the region that binds to the virus budding factor AIP-1/ALIX, indicating that this structure is required for interaction with the endosomal sorting complex required for transport. The PTAP motif at the N terminus, comprising the primary late assembly domain, which is crucial for interaction with another cellular budding factor, Tsg101, does not exhibit secondary structure. However, the adjacent helix-1 may play an indirect role in the specific complex formation between p6 and the binding groove in Tsg101. Moreover, binding studies by NMR demonstrate that helix-2, which also comprises the LXXLF motif required for incorporation of the human immunodeficiency virus type 1 accessory protein Vpr into budding virions, specifically interacts with the Vpr binding region, indicating that under the specific solution conditions used for structure analysis, p6 adopted a functional conformation.
Solution structure of the human immunodeficiency virus type 1 p6 protein.,Fossen T, Wray V, Bruns K, Rachmat J, Henklein P, Tessmer U, Maczurek A, Klinger P, Schubert U J Biol Chem. 2005 Dec 30;280(52):42515-27. Epub 2005 Oct 17. PMID:16234236[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fossen T, Wray V, Bruns K, Rachmat J, Henklein P, Tessmer U, Maczurek A, Klinger P, Schubert U. Solution structure of the human immunodeficiency virus type 1 p6 protein. J Biol Chem. 2005 Dec 30;280(52):42515-27. Epub 2005 Oct 17. PMID:16234236 doi:10.1074/jbc.M507375200
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