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| | <StructureSection load='4ur6' size='340' side='right'caption='[[4ur6]], [[Resolution|resolution]] 1.20Å' scene=''> | | <StructureSection load='4ur6' size='340' side='right'caption='[[4ur6]], [[Resolution|resolution]] 1.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ur6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Blennius_viviparus Blennius viviparus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UR6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UR6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ur6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Zoarces_viviparus Zoarces viviparus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UR6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UR6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ur4|4ur4]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ur6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ur6 OCA], [http://pdbe.org/4ur6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ur6 RCSB], [http://www.ebi.ac.uk/pdbsum/4ur6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ur6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ur6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ur6 OCA], [https://pdbe.org/4ur6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ur6 RCSB], [https://www.ebi.ac.uk/pdbsum/4ur6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ur6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/R9S083_ZOAVI R9S083_ZOAVI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Blennius viviparus]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Leggio, L Lo]] | + | [[Category: Zoarces viviparus]] |
| - | [[Category: Poulsen, J C.N]] | + | [[Category: Lo Leggio L]] |
| - | [[Category: Ramloev, H]] | + | [[Category: Poulsen J-CN]] |
| - | [[Category: Wilkens, C]] | + | [[Category: Ramloev H]] |
| - | [[Category: Antifreeze protein]]
| + | [[Category: Wilkens C]] |
| - | [[Category: Fish]]
| + | |
| - | [[Category: Sp isoform]]
| + | |
| - | [[Category: Type iii]]
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| Structural highlights
Function
R9S083_ZOAVI
Publication Abstract from PubMed
Antifreeze proteins (AFPs) are essential components of many organisms adaptation to cold temperatures. Fish type III AFPs are divided into two groups, SP isoforms being much less active than QAE1 isoforms. Two type III AFPs from Zoarces viviparus, a QAE1 (ZvAFP13) and an SP (ZvAFP6) isoform, are here characterized and their crystal structures determined. We conclude that the higher activity of the QAE1 isoforms cannot be attributed to single residues, but rather a combination of structural effects. Furthermore both ZvAFP6 and ZvAFP13 crystal structures have water molecules around T18 equivalent to the tetrahedral-like waters previously identified in a neutron crystal structure. Interestingly, ZvAFP6 forms dimers in the crystal, with a significant dimer interface. The presence of ZvAFP6 dimers was confirmed in solution by native electrophoresis and gel filtration. To our knowledge this is the first report of dimerization of AFP type III proteins.
Purification, crystal structure determination and functional characterization of type III antifreeze proteins from the European eelpout Zoarces viviparus.,Wilkens C, Poulsen JC, Ramlov H, Leggio LL Cryobiology. 2014 Jul 12. pii: S0011-2240(14)00133-3. doi:, 10.1016/j.cryobiol.2014.07.003. PMID:25025819[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wilkens C, Poulsen JC, Ramlov H, Leggio LL. Purification, crystal structure determination and functional characterization of type III antifreeze proteins from the European eelpout Zoarces viviparus. Cryobiology. 2014 Jul 12. pii: S0011-2240(14)00133-3. doi:, 10.1016/j.cryobiol.2014.07.003. PMID:25025819 doi:http://dx.doi.org/10.1016/j.cryobiol.2014.07.003
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