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| | ==Electron Cryo-microscopy of Venezuelan Equine Encephalitis Virus TC- 83 in complex with neutralizing antibody Fab F5== | | ==Electron Cryo-microscopy of Venezuelan Equine Encephalitis Virus TC- 83 in complex with neutralizing antibody Fab F5== |
| - | <StructureSection load='4uom' size='340' side='right'caption='[[4uom]], [[Resolution|resolution]] 17.00Å' scene=''> | + | <SX load='4uom' size='340' side='right' viewer='molstar' caption='[[4uom]], [[Resolution|resolution]] 17.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4uom]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UOM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UOM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4uom]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UOM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UOM FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uok|4uok]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uom OCA], [https://pdbe.org/4uom PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uom RCSB], [https://www.ebi.ac.uk/pdbsum/4uom PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uom ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uom FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uom OCA], [http://pdbe.org/4uom PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uom RCSB], [http://www.ebi.ac.uk/pdbsum/4uom PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uom ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Blair, C D]] | + | [[Category: Blair CD]] |
| - | [[Category: Jose, J]] | + | [[Category: Jose J]] |
| - | [[Category: Kuhn, R J]] | + | [[Category: Kuhn RJ]] |
| - | [[Category: Porta, J]] | + | [[Category: Porta J]] |
| - | [[Category: Roehrig, J T]] | + | [[Category: Roehrig JT]] |
| - | [[Category: Rossmann, M G]] | + | [[Category: Rossmann MG]] |
| - | [[Category: Alphavirus]]
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| - | [[Category: Antibody neutralization]]
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| - | [[Category: Fab]]
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| - | [[Category: Venezuelan]]
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| - | [[Category: Viral protein]]
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| Structural highlights
Publication Abstract from PubMed
Alphaviruses are serious, sometimes lethal human pathogens that belong to the family Togaviridae. The structures of human Venezuelan equine encephalitis virus (VEEV), an alphavirus, in complex with two strongly neutralizing antibody Fab fragments (F5 and 3B4C-4) have been determined using a combination of cryo-electron microscopy and homology modeling. We characterize these monoclonal antibody Fab fragments, which are known to abrogate VEEV infectivity by binding to the E2 (envelope) surface glycoprotein. Both of these antibody Fab fragments cross-link the surface E2 glycoproteins and therefore probably inhibit infectivity by blocking the conformational changes that are required for making the virus fusogenic. The F5 Fab fragment cross-links E2 proteins within one trimeric spike, whereas the 3B4C-4 Fab fragment cross-links E2 proteins from neighboring spikes. Furthermore, F5 probably blocks the receptor-binding site, whereas 3B4C-4 sterically hinders the exposure of the fusion loop at the end of the E2 B-domain. IMPORTANCE: Alphaviral infections are transmitted mainly by mosquitoes. Venezuelan equine encephalitis virus (VEEV) is an alphavirus with a wide distribution across the globe. No effective vaccines exist for alphaviral infections. Therefore, a better understanding of VEEV and its associated neutralizing antibodies will help with the development of effective drugs and vaccines.
Locking and blocking the viral landscape of an alphavirus with neutralizing antibodies.,Porta J, Jose J, Roehrig JT, Blair CD, Kuhn RJ, Rossmann MG J Virol. 2014 Sep 1;88(17):9616-23. doi: 10.1128/JVI.01286-14. Epub 2014 Jun 11. PMID:24920796[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Porta J, Jose J, Roehrig JT, Blair CD, Kuhn RJ, Rossmann MG. Locking and blocking the viral landscape of an alphavirus with neutralizing antibodies. J Virol. 2014 Sep 1;88(17):9616-23. doi: 10.1128/JVI.01286-14. Epub 2014 Jun 11. PMID:24920796 doi:http://dx.doi.org/10.1128/JVI.01286-14
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