6m0w

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'''Unreleased structure'''
 
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The entry 6m0w is ON HOLD
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==Crystal structure of Streptococcus thermophilus Cas9 in complex with the AGAA PAM==
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<StructureSection load='6m0w' size='340' side='right'caption='[[6m0w]], [[Resolution|resolution]] 2.76&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6m0w]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_thermophilus Streptococcus thermophilus] and [https://en.wikipedia.org/wiki/Streptococcus_thermophilus_LMD-9 Streptococcus thermophilus LMD-9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M0W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.76&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m0w OCA], [https://pdbe.org/6m0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m0w RCSB], [https://www.ebi.ac.uk/pdbsum/6m0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m0w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAS9A_STRTD CAS9A_STRTD] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity). Cuts target DNA when Cas9 and gRNAs are mixed.[HAMAP-Rule:MF_01480]<ref>PMID:24270795</ref>
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Authors: Zhang, Y., Zhang, H., Xu, X., Wang, Y., Chen, W., Wang, Y., Wu, Z., Tang, N., Wang, Y., Zhao, S., Gan, J., Ji, Q.
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==See Also==
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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Description: Crystal structure of Streptococcus thermophilus Cas9 in complex with the AGAA PAM
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== References ==
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[[Category: Unreleased Structures]]
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<references/>
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[[Category: Zhang, H]]
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__TOC__
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[[Category: Zhao, S]]
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</StructureSection>
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[[Category: Wu, Z]]
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[[Category: Large Structures]]
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[[Category: Wang, Y]]
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[[Category: Streptococcus thermophilus]]
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[[Category: Zhang, Y]]
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[[Category: Streptococcus thermophilus LMD-9]]
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[[Category: Ji, Q]]
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[[Category: Chen W]]
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[[Category: Gan, J]]
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[[Category: Gan J]]
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[[Category: Chen, W]]
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[[Category: Ji Q]]
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[[Category: Tang, N]]
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[[Category: Tang N]]
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[[Category: Xu, X]]
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[[Category: Wang Y]]
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[[Category: Wu Z]]
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[[Category: Xu X]]
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[[Category: Zhang H]]
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[[Category: Zhang Y]]
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[[Category: Zhao S]]

Current revision

Crystal structure of Streptococcus thermophilus Cas9 in complex with the AGAA PAM

PDB ID 6m0w

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OCA

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