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5ach

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X-ray Structure of LPMO

Structural highlights

5ach is a 1 chain structure with sequence from Panus similis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.28Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A0S2GKZ1_9APHY

Publication Abstract from PubMed

Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that oxidatively break down recalcitrant polysaccharides such as cellulose and chitin. Since their discovery, LPMOs have become integral factors in the industrial utilization of biomass, especially in the sustainable generation of cellulosic bioethanol. We report here a structural determination of an LPMO-oligosaccharide complex, yielding detailed insights into the mechanism of action of these enzymes. Using a combination of structure and electron paramagnetic resonance spectroscopy, we reveal the means by which LPMOs interact with saccharide substrates. We further uncover electronic and structural features of the enzyme active site, showing how LPMOs orchestrate the reaction of oxygen with polysaccharide chains.

The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases.,Frandsen KE, Simmons TJ, Dupree P, Poulsen JN, Hemsworth GR, Ciano L, Johnston EM, Tovborg M, Johansen KS, von Freiesleben P, Marmuse L, Fort S, Cottaz S, Driguez H, Henrissat B, Lenfant N, Tuna F, Baldansuren A, Davies GJ, Lo Leggio L, Walton PH Nat Chem Biol. 2016 Feb 29. doi: 10.1038/nchembio.2029. PMID:26928935^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Frandsen KE, Simmons TJ, Dupree P, Poulsen JN, Hemsworth GR, Ciano L, Johnston EM, Tovborg M, Johansen KS, von Freiesleben P, Marmuse L, Fort S, Cottaz S, Driguez H, Henrissat B, Lenfant N, Tuna F, Baldansuren A, Davies GJ, Lo Leggio L, Walton PH. The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases. Nat Chem Biol. 2016 Feb 29. doi: 10.1038/nchembio.2029. PMID:26928935 doi:http://dx.doi.org/10.1038/nchembio.2029

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ach"

@@ Line 3: / Line 3: @@
 <StructureSection load='5ach' size='340' side='right'caption='[[5ach]], [[Resolution|resolution]] 1.28&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ach]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lentinus_similis Lentinus similis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ACH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ACH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ach]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Panus_similis Panus similis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ACH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ACH FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.28&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5acf|5acf]], [[5acg|5acg]], [[5aci|5aci]], [[5acj|5acj]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ach FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ach OCA], [https://pdbe.org/5ach PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ach RCSB], [https://www.ebi.ac.uk/pdbsum/5ach PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ach ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ach FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ach OCA], [http://pdbe.org/5ach PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ach RCSB], [http://www.ebi.ac.uk/pdbsum/5ach PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ach ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A0S2GKZ1_9APHY A0A0S2GKZ1_9APHY]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 26: / Line 27: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Lentinus similis]]
+[[Category: Panus similis]]
-[[Category: Frandsen, K E.H]]
+[[Category: Frandsen KEH]]
-[[Category: Johansen, K S]]
+[[Category: Johansen KS]]
-[[Category: Leggio, L Lo]]
+[[Category: Lo Leggio L]]
-[[Category: Poulsen, J N]]
+[[Category: Poulsen JN]]
-[[Category: Tovborg, M]]
+[[Category: Tovborg M]]
-[[Category: Monooxygenase]]
-[[Category: Oxidoreductase]]

5ach

From Proteopedia

Current revision

X-ray Structure of LPMO

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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