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| | <StructureSection load='2ddc' size='340' side='right'caption='[[2ddc]], [[Resolution|resolution]] 1.55Å' scene=''> | | <StructureSection load='2ddc' size='340' side='right'caption='[[2ddc]], [[Resolution|resolution]] 1.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2ddc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Favfa Favfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DDC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DDC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2ddc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dipsastraea_favus Dipsastraea favus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DDC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DDC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ddd|2ddd]]</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ddc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ddc OCA], [https://pdbe.org/2ddc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ddc RCSB], [https://www.ebi.ac.uk/pdbsum/2ddc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ddc ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ddc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ddc OCA], [http://pdbe.org/2ddc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ddc RCSB], [http://www.ebi.ac.uk/pdbsum/2ddc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ddc ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q53UG8_DIPFA Q53UG8_DIPFA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Favfa]] | + | [[Category: Dipsastraea favus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Miyawaki, A]] | + | [[Category: Miyawaki A]] |
| - | [[Category: Nukina, N]] | + | [[Category: Nukina N]] |
| - | [[Category: Shimizu, H]] | + | [[Category: Shimizu H]] |
| - | [[Category: Tsutsui, H]] | + | [[Category: Tsutsui H]] |
| - | [[Category: Luminescent protein]]
| + | |
| - | [[Category: Photoconversion]]
| + | |
| - | [[Category: Red state]]
| + | |
| Structural highlights
Function
Q53UG8_DIPFA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
KikGR is a fluorescent protein engineered to display green-to-red photoconvertibility that is induced by irradiation with ultraviolet or violet light. Similar to Kaede and EosFP, two naturally occurring photoconvertible proteins, KikGR contains a His(62)-Tyr(63)-Gly(64) tripeptide sequence, which forms a green chromophore that can be photoconverted to a red one via formal beta-elimination and subsequent extension of a pi-conjugated system. Using a crystallizable variant of KikGR, we determined the structures of both the green and red state at 1.55 A resolution. The double bond between His(62)-C(alpha) and His(62)-C(beta) in the red chromophore is in a cis configuration, indicating that rotation along the His(62) C(alpha)-C(beta) bond occurs following cleavage of the His(62) N(alpha)-C(alpha) bond. This structural rearrangement provides evidence that the beta-elimination reaction governing the green-to-red photoconversion of KikGR follows an E1 (elimination, unimolecular) mechanism.
The E1 mechanism in photo-induced beta-elimination reactions for green-to-red conversion of fluorescent proteins.,Tsutsui H, Shimizu H, Mizuno H, Nukina N, Furuta T, Miyawaki A Chem Biol. 2009 Nov 25;16(11):1140-7. PMID:19942137[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Tsutsui H, Shimizu H, Mizuno H, Nukina N, Furuta T, Miyawaki A. The E1 mechanism in photo-induced beta-elimination reactions for green-to-red conversion of fluorescent proteins. Chem Biol. 2009 Nov 25;16(11):1140-7. PMID:19942137 doi:10.1016/j.chembiol.2009.10.010
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