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4xoh

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Mechanistic insights into anchorage of the contractile ring from yeast to humans

Structural highlights

4xoh is a 3 chain structure with sequence from Schizosaccharomyces pombe 972h-. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.801Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MID1_SCHPO At the onset of mitosis, forms a medial ring structure before the arrangement of the medial actin ring. Essential for the central positioning of the division septum before the cell divides.^[1] ^[2]

Publication Abstract from PubMed

Anillins and Mid1 are scaffold proteins that play key roles in anchorage of the contractile ring at the cell equator during cytokinesis in animals and fungi, respectively. Here, we report crystal structures and functional analysis of human anillin and S. pombe Mid1. The combined data show anillin contains a cryptic C2 domain and a Rho-binding domain. Together with the tethering PH domain, three membrane-associating elements synergistically bind to RhoA and phospholipids to anchor anillin at the cleavage furrow. Surprisingly, Mid1 also binds to the membrane through a cryptic C2 domain. Dimerization of Mid1 leads to high affinity and preference for PI(4,5)P2, which stably anchors Mid1 at the division plane, bypassing the requirement for Rho GTPase. These findings uncover the unexpected general machinery and the divergent regulatory logics for the anchorage of the contractile ring through the anillin/Mid1 family proteins from yeast to humans.

Mechanistic insights into the anchorage of the contractile ring by anillin and mid1.,Sun L, Guan R, Lee IJ, Liu Y, Chen M, Wang J, Wu JQ, Chen Z Dev Cell. 2015 May 26;33(4):413-26. doi: 10.1016/j.devcel.2015.03.003. Epub 2015 , May 7. PMID:25959226^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Moseley JB, Mayeux A, Paoletti A, Nurse P. A spatial gradient coordinates cell size and mitotic entry in fission yeast. Nature. 2009 Jun 11;459(7248):857-60. doi: 10.1038/nature08074. Epub 2009 May 27. PMID:19474789 doi:http://dx.doi.org/10.1038/nature08074
↑ Sohrmann M, Fankhauser C, Brodbeck C, Simanis V. The dmf1/mid1 gene is essential for correct positioning of the division septum in fission yeast. Genes Dev. 1996 Nov 1;10(21):2707-19. PMID:8946912
↑ Sun L, Guan R, Lee IJ, Liu Y, Chen M, Wang J, Wu JQ, Chen Z. Mechanistic insights into the anchorage of the contractile ring by anillin and mid1. Dev Cell. 2015 May 26;33(4):413-26. doi: 10.1016/j.devcel.2015.03.003. Epub 2015 , May 7. PMID:25959226 doi:http://dx.doi.org/10.1016/j.devcel.2015.03.003

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xoh"

@@ Line 3: / Line 3: @@
 <StructureSection load='4xoh' size='340' side='right'caption='[[4xoh]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4xoh]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XOH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XOH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4xoh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XOH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XOH FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xh3|4xh3]], [[4xoi|4xoi]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.801&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xoh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xoh OCA], [http://pdbe.org/4xoh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xoh RCSB], [http://www.ebi.ac.uk/pdbsum/4xoh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xoh ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xoh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xoh OCA], [https://pdbe.org/4xoh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xoh RCSB], [https://www.ebi.ac.uk/pdbsum/4xoh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xoh ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/MID1_SCHPO MID1_SCHPO]] At the onset of mitosis, forms a medial ring structure before the arrangement of the medial actin ring. Essential for the central positioning of the division septum before the cell divides.<ref>PMID:19474789</ref> <ref>PMID:8946912</ref>
+[https://www.uniprot.org/uniprot/MID1_SCHPO MID1_SCHPO] At the onset of mitosis, forms a medial ring structure before the arrangement of the medial actin ring. Essential for the central positioning of the division septum before the cell divides.<ref>PMID:19474789</ref> <ref>PMID:8946912</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Chen, M]]
+[[Category: Schizosaccharomyces pombe 972h-]]
-[[Category: Chen, Z]]
+[[Category: Chen M]]
-[[Category: Guan, R]]
+[[Category: Chen Z]]
-[[Category: Lee, I J]]
+[[Category: Guan R]]
-[[Category: Liu, Y]]
+[[Category: Lee I-J]]
-[[Category: Sun, L]]
+[[Category: Liu Y]]
-[[Category: Wang, J]]
+[[Category: Sun L]]
-[[Category: Wu, J Q]]
+[[Category: Wang J]]
-[[Category: Cell cycle]]
+[[Category: Wu J-Q]]
-[[Category: Mid1]]

4xoh

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Current revision

Mechanistic insights into anchorage of the contractile ring from yeast to humans

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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