4ypj

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<StructureSection load='4ypj' size='340' side='right'caption='[[4ypj]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='4ypj' size='340' side='right'caption='[[4ypj]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ypj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YPJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YPJ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ypj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YPJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YPJ FirstGlance]. <br>
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</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ypj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ypj OCA], [http://pdbe.org/4ypj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ypj RCSB], [http://www.ebi.ac.uk/pdbsum/4ypj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ypj ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ypj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ypj OCA], [https://pdbe.org/4ypj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ypj RCSB], [https://www.ebi.ac.uk/pdbsum/4ypj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ypj ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/E5RWQ2_NIACI E5RWQ2_NIACI]
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beta-galactosidase (EC3.2.1.23) from Bacillus circulans ATCC 31382, designated BgaD, exhibits high transglycosylation activity to produce galacto-oligosaccharides. BgaD has been speculated to have a multiple domain architecture including a F5/8 type C domain or a discoidin (DS) domain in the C-terminal peptide region from amino acid sequence analysis. Here, we solved the first crystal structure of the C-terminal deletion mutant BgaD-D, consisting of sugar binding, Glyco_hydro, catalytic, and bacterial Ig-like domains, at 2.5 A. In the asymmetric unit, two molecules of BgaD-D were identified and the value of VM was estimated to be 5.0 A3 /Da. It had been speculated that the structure of BgaD-D consists of four domains. From the structural analysis, however, we clarified that BgaD-D consists of five domains. We identified a new domain structure comprised of beta-sheets in BgaD. The catalytic domain exhibits a TIM barrel structure with a small pocket suited for accommodating the disaccharides. Detailed structural information for the amino acid residues related to activity and substrate specificity was clarified in the catalytic domain. Furthermore, using the structural information, we successfully constructed some thermostable mutants via protein engineering method. This article is protected by copyright. All rights reserved.
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Crystal structure of beta-galactosidase from Bacillus circulans ATCC 31382 (BgaD) and the construction of the thermophilic mutants.,Ishikawa K, Kataoka M, Yanamoto T, Nakabayashi M, Watanabe M, Ishihara S, Yamaguchi S FEBS J. 2015 Apr 16. doi: 10.1111/febs.13298. PMID:25879162<ref>PMID:25879162</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ypj" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Galactosidase 3D structures|Galactosidase 3D structures]]
*[[Galactosidase 3D structures|Galactosidase 3D structures]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Beta-galactosidase]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Ishikawa, K]]
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[[Category: Niallia circulans]]
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[[Category: Kataoka, M]]
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[[Category: Ishikawa K]]
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[[Category: Nakabayashi, M]]
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[[Category: Kataoka M]]
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[[Category: Watanabe, M]]
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[[Category: Nakabayashi M]]
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[[Category: Yanamoto, T]]
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[[Category: Watanabe M]]
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[[Category: Glycoside hydrolase]]
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[[Category: Yanamoto T]]
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[[Category: Hydrolase]]
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Current revision

X-ray Structure of The Mutant of Glycoside Hydrolase

PDB ID 4ypj

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