This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3jao

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:42, 7 February 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 3: Line 3:
<SX load='3jao' size='340' side='right' viewer='molstar' caption='[[3jao]], [[Resolution|resolution]] 23.00&Aring;' scene=''>
<SX load='3jao' size='340' side='right' viewer='molstar' caption='[[3jao]], [[Resolution|resolution]] 23.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3jao]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAO FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3jao]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JAO FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 23&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jao OCA], [http://pdbe.org/3jao PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3jao RCSB], [http://www.ebi.ac.uk/pdbsum/3jao PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3jao ProSAT]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jao OCA], [https://pdbe.org/3jao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jao RCSB], [https://www.ebi.ac.uk/pdbsum/3jao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jao ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A0A0M3KKT1_TETTH A0A0M3KKT1_TETTH] Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.[RuleBase:RU000352]
-
Microtubule doublet (MTD) is the main skeleton of cilia/flagella. Many proteins, such as dyneins and radial spokes, bind to MTD, and generate or regulate force. While the structure of the reconstituted microtubule has been solved at atomic resolution, nature of the axonemal MTD is still unclear. There are a few hypotheses of the lattice arrangement of its alpha- and beta-tubulins, but it has not been described how dyneins and radial spokes bind to MTD. In this study, we analyzed the three-dimensional structure of Tetrahymena MTD at approximately 19 A resolution by single particle cryo-electron microscopy. To identify alpha- and beta-tubulins, we combined image analysis of MTD with specific kinesin decoration. This work reveals that alpha- and beta-tubulins form a B-lattice arrangement in the entire MTD with a seam at the outer junction. We revealed the unique way in which inner arm dyneins, radial spokes, and proteins inside MTD bind and bridge protofilaments.
+
-
 
+
-
alpha- and beta-Tubulin Lattice of the Axonemal Microtubule Doublet and Binding Proteins Revealed by Single Particle Cryo-Electron Microscopy and Tomography.,Maheshwari A, Obbineni JM, Bui KH, Shibata K, Toyoshima YY, Ishikawa T Structure. 2015 Jul 23. pii: S0969-2126(15)00270-1. doi:, 10.1016/j.str.2015.06.017. PMID:26211611<ref>PMID:26211611</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 3jao" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</SX>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Tetrahymena thermophila]]
[[Category: Tetrahymena thermophila]]
-
[[Category: Bui, K H]]
+
[[Category: Bui KH]]
-
[[Category: Ishikawa, T]]
+
[[Category: Ishikawa T]]
-
[[Category: Maheshwari, A]]
+
[[Category: Maheshwari A]]
-
[[Category: Obbineni, J M]]
+
[[Category: Obbineni JM]]
-
[[Category: Shibata, K]]
+
[[Category: Shibata K]]
-
[[Category: Toyoshima, Y Y]]
+
[[Category: Toyoshima YY]]
-
[[Category: Cilia]]
+
-
[[Category: Microtubule doublet]]
+
-
[[Category: Structural protein]]
+
-
[[Category: Tubulin]]
+

Current revision

Ciliary microtubule doublet

3jao, resolution 23.00Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3jao"
Personal tools