6imm

From Proteopedia

(Difference between revisions)

Current revision

Cryo-EM structure of an alphavirus, Sindbis virus

6imm, resolution 3.50Å

Structural highlights

6imm is a 12 chain structure with sequence from Sindbis virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.5Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A3G2BZ29_SINDV

Publication Abstract from PubMed

Alphaviruses are enveloped RNA viruses that contain several human pathogens. Due to intrinsic heterogeneity of alphavirus particles, a high resolution structure of the virion is currently lacking. Here we provide a 3.5 A cryo-EM structure of Sindbis virus, using block based reconstruction method that overcomes the heterogeneity problem. Our structural analysis identifies a number of conserved residues that play pivotal roles in the virus life cycle. We identify a hydrophobic pocket in the subdomain D of E2 protein that is stabilized by an unknown pocket factor near the viral membrane. Residues in the pocket are conserved in different alphaviruses. The pocket strengthens the interactions of the E1/E2 heterodimer and may facilitate virus assembly. Our study provides structural insights into alphaviruses that may inform the design of drugs and vaccines.

Implication for alphavirus host-cell entry and assembly indicated by a 3.5A resolution cryo-EM structure.,Chen L, Wang M, Zhu D, Sun Z, Ma J, Wang J, Kong L, Wang S, Liu Z, Wei L, He Y, Wang J, Zhang X Nat Commun. 2018 Dec 14;9(1):5326. doi: 10.1038/s41467-018-07704-x. PMID:30552337^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen L, Wang M, Zhu D, Sun Z, Ma J, Wang J, Kong L, Wang S, Liu Z, Wei L, He Y, Wang J, Zhang X. Implication for alphavirus host-cell entry and assembly indicated by a 3.5A resolution cryo-EM structure. Nat Commun. 2018 Dec 14;9(1):5326. doi: 10.1038/s41467-018-07704-x. PMID:30552337 doi:http://dx.doi.org/10.1038/s41467-018-07704-x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6imm"

Categories: Large Structures | Sindbis virus | Chen L | Ma J | Zhang X

@@ Line 3: / Line 3: @@
 <SX load='6imm' size='340' side='right' viewer='molstar' caption='[[6imm]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6imm]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Sindbis_virus Sindbis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IMM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IMM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6imm]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Sindbis_virus Sindbis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IMM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IMM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8K6:OCTADECANE'>8K6</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6imm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6imm OCA], [http://pdbe.org/6imm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6imm RCSB], [http://www.ebi.ac.uk/pdbsum/6imm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6imm ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8K6:OCTADECANE'>8K6</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6imm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6imm OCA], [https://pdbe.org/6imm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6imm RCSB], [https://www.ebi.ac.uk/pdbsum/6imm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6imm ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A3G2BZ29_SINDV A0A3G2BZ29_SINDV]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 25: @@
 [[Category: Large Structures]]
 [[Category: Sindbis virus]]
-[[Category: Chen, L]]
+[[Category: Chen L]]
-[[Category: Ma, J]]
+[[Category: Ma J]]
-[[Category: Zhang, X]]
+[[Category: Zhang X]]
-[[Category: Alphavirus]]
-[[Category: Glycoprotein]]
-[[Category: Virus]]

6imm

From Proteopedia

Current revision

Cryo-EM structure of an alphavirus, Sindbis virus

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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