|
|
| (10 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1ao2.gif|left|200px]] | |
| | | | |
| - | <!--
| + | ==cobalt(III)-deglycopepleomycin determined by NMR studies== |
| - | The line below this paragraph, containing "STRUCTURE_1ao2", creates the "Structure Box" on the page.
| + | <StructureSection load='1ao2' size='340' side='right'caption='[[1ao2]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AO2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AO2 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ao2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ao2 OCA], [https://pdbe.org/1ao2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ao2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ao2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ao2 ProSAT]</span></td></tr> |
| - | -->
| + | </table> |
| - | {{STRUCTURE_1ao2| PDB=1ao2 | SCENE= }}
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | '''cobalt(III)-deglycopepleomycin determined by NMR studies'''
| + | [[Category: Large Structures]] |
| - | | + | [[Category: Caceres-Cortes J]] |
| - | | + | [[Category: Ikudome K]] |
| - | ==Overview== | + | [[Category: Saito I]] |
| - | Pepleomycin (PEP) is a metalloglycopeptide that has stronger anticancer activity and less pulmonary toxicity than bleomycin (BLM). PEP, like BLM, exerts its action by binding to and degrading DNA in the presence of oxygen and certain metals. Obtaining detailed structural information of PEP and PEP-DNA complexes is crucial to understanding its anticancer activity. The structures of two green forms of cobalt-PEP species, HO2-Co(III)-PEP (denoted CoPEP) and deglycosylated HO2-Co(III)-PEP (denoted CodPEP) have been obtained by NOE restrained refinements. Earlier studies of the related HO2-Co(III)-BLM A2 proposed that two chiral conformers (form A or B) could exist with either the beta-aminoalanine primary amine (A,NH2) or the mannose carbamoyl nitrogen (M,NH2) as the axial ligand. Analysis of our NOESY data shows convincingly that form A is the most probable conformer with the mannose carbamoyl M,NH2 and the beta-aminoalanine primary amine A,NH2 as the axial ligands in CoPEP and CodPEP, respectively. The NOE cross-peaks resulting from the interactions between the N-terminus (i.e., the metal-binding domain) and the C-terminus of CoPEP and CodPEP have similar patterns, suggesting that they both adopt compact structures with the bithiazole group folded back over the N-terminus.
| + | [[Category: Sugiyama H]] |
| - | | + | [[Category: Wang AH-J]] |
| - | ==About this Structure== | + | |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AO2 OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | Structures of cobalt(III)-pepleomycin and cobalt(III)-deglycopepleomycin (green forms) determined by NMR studies., Caceres-Cortes J, Sugiyama H, Ikudome K, Saito I, Wang AH, Eur J Biochem. 1997 Mar 15;244(3):818-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9108252 9108252]
| + | |
| - | [[Category: Caceres-Cortes, J.]] | + | |
| - | [[Category: Ikudome, K.]] | + | |
| - | [[Category: Saito, I.]]
| + | |
| - | [[Category: Sugiyama, H.]]
| + | |
| - | [[Category: Wang, A H.J.]] | + | |
| - | [[Category: Anticancer drug]]
| + | |
| - | [[Category: Bleomycin]] | + | |
| - | [[Category: Deglycosylated pepleomycin]] | + | |
| - | [[Category: Dna]] | + | |
| - | [[Category: Peplomycin]] | + | |
| - | [[Category: Solution structure]] | + | |
| - | [[Category: Two-dimensional nmr]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:29:48 2008''
| + | |
