4c3g

From Proteopedia

(Difference between revisions)

Current revision

cryo-EM structure of activated and oligomeric restriction endonuclease SgrAI

4c3g, resolution 8.60Å

Structural highlights

4c3g is a 6 chain structure with sequence from Streptomyces griseus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 8.6Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9F6L0_STRGR

Publication Abstract from PubMed

SgrAI is a sequence specific DNA endonuclease that functions through an unusual enzymatic mechanism that is allosterically activated 200- to 500-fold by effector DNA, with a concomitant expansion of its DNA sequence specificity. Using single-particle transmission electron microscopy to reconstruct distinct populations of SgrAI oligomers, we show that in the presence of allosteric, activating DNA, the enzyme forms regular, repeating helical structures characterized by the addition of DNA-binding dimeric SgrAI subunits in a run-on manner. We also present the structure of oligomeric SgrAI at 8.6 A resolution, demonstrating the conformational state of SgrAI in its activated form. Activated and oligomeric SgrAI displays key protein-protein interactions near the helix axis between its N termini, as well as allosteric protein-DNA interactions that are required for enzymatic activation. The hybrid approach reveals an unusual mechanism of enzyme activation that explains SgrAI's oligomerization and allosteric behavior.

Allosteric Regulation of DNA Cleavage and Sequence-Specificity through Run-On Oligomerization.,Lyumkis D, Talley H, Stewart A, Shah S, Park CK, Tama F, Potter CS, Carragher B, Horton NC Structure. 2013 Sep 17. pii: S0969-2126(13)00304-3. doi:, 10.1016/j.str.2013.08.012. PMID:24055317^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lyumkis D, Talley H, Stewart A, Shah S, Park CK, Tama F, Potter CS, Carragher B, Horton NC. Allosteric Regulation of DNA Cleavage and Sequence-Specificity through Run-On Oligomerization. Structure. 2013 Sep 17. pii: S0969-2126(13)00304-3. doi:, 10.1016/j.str.2013.08.012. PMID:24055317 doi:http://dx.doi.org/10.1016/j.str.2013.08.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4c3g"

@@ Line 3: / Line 3: @@
 <SX load='4c3g' size='340' side='right' viewer='molstar' caption='[[4c3g]], [[Resolution|resolution]] 8.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4c3g]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseus"_krainsky_1914 "actinomyces griseus" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C3G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4c3g]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C3G FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3g OCA], [http://pdbe.org/4c3g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c3g RCSB], [http://www.ebi.ac.uk/pdbsum/4c3g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c3g ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c3g OCA], [https://pdbe.org/4c3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c3g RCSB], [https://www.ebi.ac.uk/pdbsum/4c3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c3g ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9F6L0_STRGR Q9F6L0_STRGR]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 22: @@
 __TOC__
 </SX>
-[[Category: Actinomyces griseus krainsky 1914]]
 [[Category: Large Structures]]
-[[Category: Carragher, B]]
+[[Category: Streptomyces griseus]]
-[[Category: Horton, N C]]
+[[Category: Carragher B]]
-[[Category: Lyumkis, D]]
+[[Category: Horton NC]]
-[[Category: Park, C K]]
+[[Category: Lyumkis D]]
-[[Category: Potter, C S]]
+[[Category: Park CK]]
-[[Category: Shah, S]]
+[[Category: Potter CS]]
-[[Category: Stewart, A]]
+[[Category: Shah S]]
-[[Category: Talley, H]]
+[[Category: Stewart A]]
-[[Category: Tama, F]]
+[[Category: Talley H]]
-[[Category: Allostery]]
+[[Category: Tama F]]
-[[Category: Dna cleavage]]
-[[Category: Hydrolase]]
-[[Category: Parasite-host interaction]]
-[[Category: Restriction endonuclease]]

4c3g

From Proteopedia

Current revision

cryo-EM structure of activated and oligomeric restriction endonuclease SgrAI

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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