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| | <SX load='5hx2' size='340' side='right' viewer='molstar' caption='[[5hx2]], [[Resolution|resolution]] 3.80Å' scene=''> | | <SX load='5hx2' size='340' side='right' viewer='molstar' caption='[[5hx2]], [[Resolution|resolution]] 3.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5hx2]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HX2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HX2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5hx2]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HX2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HX2 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 53 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), 10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hx2 OCA], [http://pdbe.org/5hx2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hx2 RCSB], [http://www.ebi.ac.uk/pdbsum/5hx2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hx2 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hx2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hx2 OCA], [https://pdbe.org/5hx2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hx2 RCSB], [https://www.ebi.ac.uk/pdbsum/5hx2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hx2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BP53_BPT4 BP53_BPT4]] Baseplate protein that is part of the baseplate wedge (PubMed:15315755). Involved in the tail assembly.<ref>PMID:15315755</ref> <ref>PMID:21129200</ref> [[http://www.uniprot.org/uniprot/BP07_BPT4 BP07_BPT4]] Baseplate protein that is part of the baseplate wedge. Involved in the tail assembly.<ref>PMID:2254933</ref> <ref>PMID:21129200</ref> [[http://www.uniprot.org/uniprot/BP06_BPT4 BP06_BPT4]] Baseplate protein that is part of the baseplate wedge (PubMed:15315755). Involved in the tail assembly.<ref>PMID:15315755</ref> <ref>PMID:21129200</ref> [[http://www.uniprot.org/uniprot/BP08_BPT4 BP08_BPT4]] Baseplate protein that is part of the baseplate wedge. Involved in the tail assembly.<ref>PMID:15315755</ref> <ref>PMID:21129200</ref> [[http://www.uniprot.org/uniprot/BP10_BPT4 BP10_BPT4]] Baseplate protein that is part of the baseplate wedge and that connects the short tail fibers to the baseplate (PubMed:16554069). During infection, the baseplate undergoes a conformational change from a dome-shaped to a star-shaped structure. At this point, gp10 rotates and acts as a lever that unfolds the short tail fibers, which then interact with host cell surface receptors. Involved in the tail assembly.<ref>PMID:16554069</ref> <ref>PMID:21129200</ref> | + | [https://www.uniprot.org/uniprot/BP07_BPT4 BP07_BPT4] Baseplate protein that is part of the baseplate wedge. Involved in the tail assembly.<ref>PMID:2254933</ref> <ref>PMID:21129200</ref> |
| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Bacteriophage T4 consists of a head for protecting its genome and a sheathed tail for inserting its genome into a host. The tail terminates with a multiprotein baseplate that changes its conformation from a "high-energy" dome-shaped to a "low-energy" star-shaped structure during infection. Although these two structures represent different minima in the total energy landscape of the baseplate assembly, as the dome-shaped structure readily changes to the star-shaped structure when the virus infects a host bacterium, the dome-shaped structure must have more energy than the star-shaped structure. Here we describe the electron microscopy structure of a 3.3-MDa in vitro-assembled star-shaped baseplate with a resolution of 3.8 A. This structure, together with other genetic and structural data, shows why the high-energy baseplate is formed in the presence of the central hub and how the baseplate changes to the low-energy structure, via two steps during infection. Thus, the presence of the central hub is required to initiate the assembly of metastable, high-energy structures. If the high-energy structure is formed and stabilized faster than the low-energy structure, there will be insufficient components to assemble the low-energy structure.
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| - | Role of bacteriophage T4 baseplate in regulating assembly and infection.,Yap ML, Klose T, Arisaka F, Speir JA, Veesler D, Fokine A, Rossmann MG Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2654-9. doi:, 10.1073/pnas.1601654113. Epub 2016 Feb 29. PMID:26929357<ref>PMID:26929357</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5hx2" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Bpt4]] | + | [[Category: Escherichia virus T4]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fokine, A]] | + | [[Category: Fokine A]] |
| - | [[Category: Klose, T]] | + | [[Category: Klose T]] |
| - | [[Category: Rossmann, M G]] | + | [[Category: Rossmann MG]] |
| - | [[Category: Yap, M L]] | + | [[Category: Yap ML]] |
| - | [[Category: Baseplate]]
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| - | [[Category: Complex]]
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| - | [[Category: T4]]
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| - | [[Category: Viral protein]]
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