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| | <SX load='5jpq' size='340' side='right' viewer='molstar' caption='[[5jpq]], [[Resolution|resolution]] 7.30Å' scene=''> | | <SX load='5jpq' size='340' side='right' viewer='molstar' caption='[[5jpq]], [[Resolution|resolution]] 7.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5jpq]] is a 55 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast], [http://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [http://en.wikipedia.org/wiki/Chaetomium_thermophilum_ Chaetomium thermophilum ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JPQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JPQ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5jpq]] is a 27 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JPQ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jpq OCA], [http://pdbe.org/5jpq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jpq RCSB], [http://www.ebi.ac.uk/pdbsum/5jpq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jpq ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jpq OCA], [https://pdbe.org/5jpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jpq RCSB], [https://www.ebi.ac.uk/pdbsum/5jpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jpq ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/G0S3V7_CHATD G0S3V7_CHATD]] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.[PIRNR:PIRNR006515] [[http://www.uniprot.org/uniprot/UTP21_YEAST UTP21_YEAST]] Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.<ref>PMID:15590835</ref> | + | [https://www.uniprot.org/uniprot/G0S5L1_CHATD G0S5L1_CHATD] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Chaetomium thermophilum]] | | [[Category: Chaetomium thermophilum]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Beckmann, R]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Berninghausen, O]] | + | [[Category: Beckmann R]] |
| - | [[Category: Cheng, J]] | + | [[Category: Berninghausen O]] |
| - | [[Category: Flemming, D]] | + | [[Category: Cheng J]] |
| - | [[Category: Hurt, E]] | + | [[Category: Flemming D]] |
| - | [[Category: Kornprobst, M]] | + | [[Category: Hurt E]] |
| - | [[Category: Kos, M]] | + | [[Category: Kornprobst M]] |
| - | [[Category: Kos-Braun, I C]] | + | [[Category: Kos M]] |
| - | [[Category: Thoms, M]] | + | [[Category: Kos-Braun IC]] |
| - | [[Category: Turk, M]] | + | [[Category: Thoms M]] |
| - | [[Category: Nuclear rnp]]
| + | [[Category: Turk M]] |
| - | [[Category: Ribosome]]
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| Structural highlights
Function
G0S5L1_CHATD
Publication Abstract from PubMed
The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of approximately 70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how a network of biogenesis factors including 19 beta-propellers and large alpha-solenoid proteins engulfs the pre-rRNA. Within the 90S pre-ribosome, we identify the UTP-A, UTP-B, Mpp10-Imp3-Imp4, Bms1-Rcl1, and U3 snoRNP modules, which are organized around 5'-ETS and partially folded 18S rRNA. The U3 snoRNP is strategically positioned at the center of the 90S particle to perform its multiple tasks during pre-rRNA folding and processing. The architecture of the elusive 90S pre-ribosome gives unprecedented structural insight into the early steps of pre-rRNA maturation. Nascent rRNA that is co-transcriptionally folded and given a particular shape by encapsulation within a dedicated mold-like structure is reminiscent of how polypeptides use chaperone chambers for their protein folding.
Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.,Kornprobst M, Turk M, Kellner N, Cheng J, Flemming D, Kos-Braun I, Kos M, Thoms M, Berninghausen O, Beckmann R, Hurt E Cell. 2016 Jul 14;166(2):380-93. doi: 10.1016/j.cell.2016.06.014. PMID:27419870[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kornprobst M, Turk M, Kellner N, Cheng J, Flemming D, Kos-Braun I, Kos M, Thoms M, Berninghausen O, Beckmann R, Hurt E. Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome. Cell. 2016 Jul 14;166(2):380-93. doi: 10.1016/j.cell.2016.06.014. PMID:27419870 doi:http://dx.doi.org/10.1016/j.cell.2016.06.014
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