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| | <SX load='5o09' size='340' side='right' viewer='molstar' caption='[[5o09]], [[Resolution|resolution]] 3.60Å' scene=''> | | <SX load='5o09' size='340' side='right' viewer='molstar' caption='[[5o09]], [[Resolution|resolution]] 3.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5o09]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27091 Atcc 27091] and [http://en.wikipedia.org/wiki/Atcc_700199 Atcc 700199]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O09 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O09 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5o09]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Prosthecobacter_dejongeii Prosthecobacter dejongeii] and [https://en.wikipedia.org/wiki/Prosthecobacter_vanneervenii Prosthecobacter vanneervenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O09 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5o01|5o01]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">btubA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=48465 ATCC 27091]), btubB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=48465 ATCC 27091]), bklc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=48466 ATCC 700199])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o09 OCA], [https://pdbe.org/5o09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o09 RCSB], [https://www.ebi.ac.uk/pdbsum/5o09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o09 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o09 OCA], [http://pdbe.org/5o09 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o09 RCSB], [http://www.ebi.ac.uk/pdbsum/5o09 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o09 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8GCC5_9BACT Q8GCC5_9BACT] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5o09" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5o09" style="background-color:#fffaf0;"></div> |
| - | | |
| - | ==See Also== | |
| - | *[[Tubulin|Tubulin]] | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Atcc 27091]] | |
| - | [[Category: Atcc 700199]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bharat, T A.M]] | + | [[Category: Prosthecobacter dejongeii]] |
| - | [[Category: Deng, X]] | + | [[Category: Prosthecobacter vanneervenii]] |
| - | [[Category: Lowe, J]] | + | [[Category: Bharat TAM]] |
| - | [[Category: Bacterial cytoskeleton]] | + | [[Category: Deng X]] |
| - | [[Category: Microtubule]] | + | [[Category: Lowe J]] |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Function
Q8GCC5_9BACT
Publication Abstract from PubMed
Microtubules, the dynamic, yet stiff hollow tubes built from alphabeta-tubulin protein heterodimers, are thought to be present only in eukaryotic cells. Here, we report a 3.6-A helical reconstruction electron cryomicroscopy structure of four-stranded mini microtubules formed by bacterial tubulin-like Prosthecobacter dejongeii BtubAB proteins. Despite their much smaller diameter, mini microtubules share many key structural features with eukaryotic microtubules, such as an M-loop, alternating subunits, and a seam that breaks overall helical symmetry. Using in vitro total internal reflection fluorescence microscopy, we show that bacterial mini microtubules treadmill and display dynamic instability, another hallmark of eukaryotic microtubules. The third protein in the btub gene cluster, BtubC, previously known as "bacterial kinesin light chain," binds along protofilaments every 8 nm, inhibits BtubAB mini microtubule catastrophe, and increases rescue. Our work reveals that some bacteria contain regulated and dynamic cytomotive microtubule systems that were once thought to be only useful in much larger and sophisticated eukaryotic cells.
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability.,Deng X, Fink G, Bharat TAM, He S, Kureisaite-Ciziene D, Lowe J Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):E5950-E5958. doi:, 10.1073/pnas.1705062114. Epub 2017 Jul 3. PMID:28673988[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Deng X, Fink G, Bharat TAM, He S, Kureisaite-Ciziene D, Lowe J. Four-stranded mini microtubules formed by Prosthecobacter BtubAB show dynamic instability. Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):E5950-E5958. doi:, 10.1073/pnas.1705062114. Epub 2017 Jul 3. PMID:28673988 doi:http://dx.doi.org/10.1073/pnas.1705062114
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