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5zgb

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Cryo-EM structure of the red algal PSI-LHCR

5zgb, resolution 3.63Å

Structural highlights

5zgb is a 17 chain structure with sequence from Cyanidioschyzon merolae (strain 10d) and Cyanidioschyzon merolae strain 10d. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Activity:	Photosystem I, with EC number 1.97.1.12
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PSAC_CYAM1] Apoprotein for the two 4Fe-4S centers FA and FB of photosystem I (PSI); essential for photochemical activity. FB is the terminal electron acceptor of PSI, donating electrons to ferredoxin. The C-terminus interacts with PsaA/B/D and helps assemble the protein into the PSI complex. Required for binding of PsaD and PsaE to PSI. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. [Q85FQ6_CYAM1] May help in the organization of the PsaL subunit.[SAAS:SAAS00400928] [PSAB_CYAM1] PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin or cytochrome c6. [PSAJ_CYAM1] May help in the organization of the PsaE and PsaF subunits. [PSAA_CYAM1] PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin or cytochrome c6.

Publication Abstract from PubMed

Photosystem I (PSI) is one of the two photosystems present in oxygenic photosynthetic organisms and functions to harvest and convert light energy into chemical energy in photosynthesis. In eukaryotic algae and higher plants, PSI consists of a core surrounded by variable species and numbers of light-harvesting complex (LHC)I proteins, forming a PSI-LHCI supercomplex. Here, we report cryo-EM structures of PSI-LHCR from the red alga Cyanidioschyzon merolae in two forms, one with three Lhcr subunits attached to the side, similar to that of higher plants, and the other with two additional Lhcr subunits attached to the opposite side, indicating an ancient form of PSI-LHCI. Furthermore, the red algal PSI core showed features of both cyanobacterial and higher plant PSI, suggesting an intermediate type during evolution from prokaryotes to eukaryotes. The structure of PsaO, existing in eukaryotic organisms, was identified in the PSI core and binds three chlorophylls a and may be important in harvesting energy and in mediating energy transfer from LHCII to the PSI core under state-2 conditions. Individual attaching sites of LHCRs with the core subunits were identified, and each Lhcr was found to contain 11 to 13 chlorophylls a and 5 zeaxanthins, which are apparently different from those of LHCs in plant PSI-LHCI. Together, our results reveal unique energy transfer pathways different from those of higher plant PSI-LHCI, its adaptation to the changing environment, and the possible changes of PSI-LHCI during evolution from prokaryotes to eukaryotes.

Unique organization of photosystem I-light-harvesting supercomplex revealed by cryo-EM from a red alga.,Pi X, Tian L, Dai HE, Qin X, Cheng L, Kuang T, Sui SF, Shen JR Proc Natl Acad Sci U S A. 2018 Apr 9. pii: 1722482115. doi:, 10.1073/pnas.1722482115. PMID:29632169^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pi X, Tian L, Dai HE, Qin X, Cheng L, Kuang T, Sui SF, Shen JR. Unique organization of photosystem I-light-harvesting supercomplex revealed by cryo-EM from a red alga. Proc Natl Acad Sci U S A. 2018 Apr 9. pii: 1722482115. doi:, 10.1073/pnas.1722482115. PMID:29632169 doi:http://dx.doi.org/10.1073/pnas.1722482115

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5zgb"

@@ Line 3: / Line 3: @@
 <SX load='5zgb' size='340' side='right' viewer='molstar' caption='[[5zgb]], [[Resolution|resolution]] 3.63&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5zgb]] is a 17 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyanidioschyzon_merolae_(strain_10d) Cyanidioschyzon merolae (strain 10d)] and [http://en.wikipedia.org/wiki/Cyanidioschyzon_merolae_strain_10d Cyanidioschyzon merolae strain 10d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZGB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5zgb]] is a 17 chain structure with sequence from [http://en.wikipedia.org/wiki/Cyanidioschyzon_merolae_(strain_10d) Cyanidioschyzon merolae (strain 10d)] and [http://en.wikipedia.org/wiki/Cyanidioschyzon_merolae_strain_10d Cyanidioschyzon merolae strain 10d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZGB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZGB FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1DO:1-DODECANOL'>1DO</scene>, <scene name='pdbligand=3XQ:(2S)-2,3-dihydroxypropyl+octadecanoate'>3XQ</scene>, <scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL0:CHLOROPHYLL+A+ISOMER'>CL0</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=DGD:DIGALACTOSYL+DIACYL+GLYCEROL+(DGDG)'>DGD</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=PQN:PHYLLOQUINONE'>PQN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=ZEX:(3S,5R,6S,3S,5R,6S)+BETA-CAROTENE-3,23-DIOL'>ZEX</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1DO:1-DODECANOL'>1DO</scene>, <scene name='pdbligand=3XQ:(2S)-2,3-dihydroxypropyl+octadecanoate'>3XQ</scene>, <scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL0:CHLOROPHYLL+A+ISOMER'>CL0</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=DGD:DIGALACTOSYL+DIACYL+GLYCEROL+(DGDG)'>DGD</scene>, <scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=PQN:PHYLLOQUINONE'>PQN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=ZEX:(3S,5R,6S,3S,5R,6S)+BETA-CAROTENE-3,23-DIOL'>ZEX</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photosystem_I Photosystem I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.12 1.97.1.12] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zgb OCA], [http://pdbe.org/5zgb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zgb RCSB], [http://www.ebi.ac.uk/pdbsum/5zgb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zgb ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zgb OCA], [http://pdbe.org/5zgb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zgb RCSB], [http://www.ebi.ac.uk/pdbsum/5zgb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zgb ProSAT]</span></td></tr>
 </table>
 == Function ==

5zgb

From Proteopedia

Current revision

Cryo-EM structure of the red algal PSI-LHCR

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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