6e88

<table><tr><td colspan='2'>[[6e88]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E88 FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e88 OCA], [http://pdbe.org/6e88 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e88 RCSB], [http://www.ebi.ac.uk/pdbsum/6e88 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e88 ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/TBA2_CAEEL TBA2_CAEEL]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TBB2_CAEEL TBB2_CAEEL]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

The dynamic instability of microtubules is a conserved and fundamental mechanism in eukaryotes. Yet microtubules from different species diverge in their growth rates, lattice structures, and responses to GTP hydrolysis. Therefore, we do not know what limits microtubule growth, what determines microtubule structure, or whether the mechanisms of dynamic instability are universal. Here, we studied microtubules from the nematode C. elegans, which have strikingly fast growth rates and non-canonical lattices in vivo. Using a reconstitution approach, we discovered that C. elegans microtubules combine intrinsically fast growth with very frequent catastrophes. We solved the structure of C. elegans microtubules to 4.8 A and discovered sequence divergence in the lateral contact loops, one of which is ordered in C. elegans but unresolved in other species. We provide direct evidence that C. elegans tubulin has a higher free energy in solution and propose a model wherein the ordering of lateral contact loops activates tubulin for growth.

 

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== References ==

[[Category: Brouhard, G J]]

[[Category: Bui, K H]]

[[Category: Chaaban, S]]

[[Category: Chieh-Ting, H]]

[[Category: Jariwala, S]]

[[Category: Kollman, J]]

[[Category: Muller-Reichert, T]]

[[Category: Redemann, S]]

[[Category: Sept, D]]

[[Category: Structural protein]]