6jnf

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<SX load='6jnf' size='340' side='right' viewer='molstar' caption='[[6jnf]], [[Resolution|resolution]] 3.81&Aring;' scene=''>
<SX load='6jnf' size='340' side='right' viewer='molstar' caption='[[6jnf]], [[Resolution|resolution]] 3.81&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6jnf]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JNF FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6jnf]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JNF FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=46E:(2R)-3-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-2-(TETRADECANOYLOXY)PROPYL+TETRADECANOATE'>46E</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.81&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TOM40 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), TOM7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), TOM22 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), TOM5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), TOM6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=46E:(2R)-3-{[(S)-(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-2-(TETRADECANOYLOXY)PROPYL+TETRADECANOATE'>46E</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jnf OCA], [http://pdbe.org/6jnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jnf RCSB], [http://www.ebi.ac.uk/pdbsum/6jnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jnf ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jnf OCA], [https://pdbe.org/6jnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jnf RCSB], [https://www.ebi.ac.uk/pdbsum/6jnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jnf ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TOM5_YEAST TOM5_YEAST]] Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. TOM5 is involved in insertion of preproteins into the TOM40 translocation pore.<ref>PMID:9217162</ref> [[http://www.uniprot.org/uniprot/TOM40_YEAST TOM40_YEAST]] Channel-forming protein essential for import of protein precursors into mitochondria.<ref>PMID:10427088</ref> [[http://www.uniprot.org/uniprot/TOM22_YEAST TOM22_YEAST]] Central component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM70 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Docks TOM20 and TOM70 for interaction with the general TOM40 import pore (GIP) complex. May regulate the TOM machinery organization, stability and channel gating.<ref>PMID:10519552</ref> <ref>PMID:11276259</ref> <ref>PMID:7760834</ref> [[http://www.uniprot.org/uniprot/TOM7_YEAST TOM7_YEAST]] Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. TOM7 is involved in assembly and stability of the TOM complex.<ref>PMID:11276259</ref> [[http://www.uniprot.org/uniprot/TOM6_YEAST TOM6_YEAST]] Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. TOM6 is involved in assembly and stability of the TOM complex.<ref>PMID:11276259</ref>
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[https://www.uniprot.org/uniprot/TOM40_YEAST TOM40_YEAST] Channel-forming protein essential for import of protein precursors into mitochondria.<ref>PMID:10427088</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The translocase of the outer mitochondrial membrane (TOM) is the main entry gate for proteins(1-4). Here we use cryo-electron microscopy to report the structure of the yeast TOM core complex(5-9) at 3.8 A resolution. The structure reveals the high-resolution architecture of the translocator consisting of two Tom40 beta-barrel channels and alpha-helical transmembrane subunits, providing insight into critical features conserved in all eukaryotes(1-3). Each Tom40 beta-barrel is surrounded by small Tom subunits, and tethered by two Tom22 and one phospholipid. The N-terminal extension of Tom40 forms a helix inside the channel; mutational analysis reveals its dual role in early and late steps in biogenesis of intermembrane-space proteins in cooperation with Tom5. Each Tom40 channel possesses two precursor exit sites: Tom22, Tom40 and Tom7 guide presequence-containing preproteins to the exit in the midst of the dimer, whereas Tom5 and the Tom40 N-extension guide presequence-less preproteins to the exit at the dimer periphery.
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Structure of the mitochondrial import gate reveals distinct preprotein paths.,Araiso Y, Tsutsumi A, Qiu J, Imai K, Shiota T, Song J, Lindau C, Wenz LS, Sakaue H, Yunoki K, Kawano S, Suzuki J, Wischnewski M, Schutze C, Ariyama H, Ando T, Becker T, Lithgow T, Wiedemann N, Pfanner N, Kikkawa M, Endo T Nature. 2019 Oct 10. pii: 10.1038/s41586-019-1680-7. doi:, 10.1038/s41586-019-1680-7. PMID:31600774<ref>PMID:31600774</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6jnf" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</SX>
</SX>
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[[Category: Baker's yeast]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Araiso, Y]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Endo, T]]
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[[Category: Araiso Y]]
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[[Category: Kawano, S]]
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[[Category: Endo T]]
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[[Category: Kikkawa, M]]
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[[Category: Kawano S]]
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[[Category: Suzuki, J]]
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[[Category: Kikkawa M]]
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[[Category: Tsutsumi, A]]
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[[Category: Suzuki J]]
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[[Category: Yunoki, K]]
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[[Category: Tsutsumi A]]
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[[Category: Alpha/beta translocator]]
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[[Category: Yunoki K]]
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[[Category: Membrane protein complex]]
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[[Category: Mitochondria]]
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[[Category: Protein import]]
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[[Category: Translocase]]
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Current revision

Cryo-EM structure of the translocator of the outer mitochondrial membrane

6jnf, resolution 3.81Å

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