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| | <SX load='6qcs' size='340' side='right' viewer='molstar' caption='[[6qcs]], [[Resolution|resolution]] 3.10Å' scene=''> | | <SX load='6qcs' size='340' side='right' viewer='molstar' caption='[[6qcs]], [[Resolution|resolution]] 3.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6qcs]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/9infb 9infb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QCS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6qcs]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_B_virus Influenza B virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QCS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11520 9INFB]), PB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11520 9INFB]), PB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11520 9INFB])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qcs OCA], [https://pdbe.org/6qcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qcs RCSB], [https://www.ebi.ac.uk/pdbsum/6qcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qcs ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qcs OCA], [http://pdbe.org/6qcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qcs RCSB], [http://www.ebi.ac.uk/pdbsum/6qcs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qcs ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5V8Z9_9INFB Q5V8Z9_9INFB] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| | + | [[Category: Influenza B virus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: RNA-directed RNA polymerase]]
| + | [[Category: Cusack S]] |
| - | [[Category: Cusack, S]] | + | [[Category: Kouba T]] |
| - | [[Category: Kouba, T]] | + | |
| - | [[Category: Influenza polzmerase]]
| + | |
| - | [[Category: Viral protein]]
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| Structural highlights
Function
Q5V8Z9_9INFB
Publication Abstract from PubMed
Influenza virus RNA-dependent RNA polymerase uses unique mechanisms to transcribe its single-stranded genomic viral RNA (vRNA) into messenger RNA. The polymerase is initially bound to a promoter comprising the partially base-paired 3' and 5' extremities of the RNA. A short, capped primer, 'cap-snatched' from a nascent host polymerase II transcript, is directed towards the polymerase active site to initiate RNA synthesis. Here we present structural snapshots, as determined by X-ray crystallography and cryo-electron microscopy, of actively initiating influenza polymerase as it transitions towards processive elongation. Unexpected conformational changes unblock the active site cavity to allow establishment of a nine-base-pair template-product RNA duplex before the strands separate into distinct exit channels. Concomitantly, as the template translocates, the promoter base pairs are broken and the template entry region is remodeled. These structures reveal details of the influenza polymerase active site that will help optimize nucleoside analogs or other compounds that directly inhibit viral RNA synthesis.
Structural snapshots of actively transcribing influenza polymerase.,Kouba T, Drncova P, Cusack S Nat Struct Mol Biol. 2019 Jun;26(6):460-470. doi: 10.1038/s41594-019-0232-z. Epub, 2019 Jun 3. PMID:31160782[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kouba T, Drncova P, Cusack S. Structural snapshots of actively transcribing influenza polymerase. Nat Struct Mol Biol. 2019 Jun;26(6):460-470. doi: 10.1038/s41594-019-0232-z. Epub, 2019 Jun 3. PMID:31160782 doi:http://dx.doi.org/10.1038/s41594-019-0232-z
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