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6sco

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Cryo-EM Structure of Potato Leaf Roll Virus VLP

6sco, resolution 3.30Å

Structural highlights

6sco is a 3 chain structure with sequence from Potato leafroll virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.3Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAPSD_PLRV1 Major capsid protein that self-assembles to form an icosahedral capsid with a T=3 symmetry, about 23 nm in diameter, and consisting of 180 capsid proteins monomers (PubMed:31611039, PubMed:34813955). Most of the 180 monomers are the major capsid protein, but a small percentage contain the minor capsid protein, which has a long C-terminal extension (PubMed:31611039).^[1] ^[2]

Publication Abstract from PubMed

The Luteoviridae are pathogenic plant viruses responsible for significant crop losses worldwide. They infect a wide range of food crops, including cereals, legumes, cucurbits, sugar beet, sugarcane, and potato and, as such, are a major threat to global food security. Viral replication is strictly limited to the plant vasculature, and this phloem limitation, coupled with the need for aphid transmission of virus particles, has made it difficult to generate virus in the quantities needed for high-resolution structural studies. Here, we exploit recent advances in heterologous expression in plants to produce sufficient quantities of virus-like particles for structural studies. We have determined their structures to high resolution by cryoelectron microscopy, providing the molecular-level insight required to rationally interrogate luteovirid capsid formation and aphid transmission, thereby providing a platform for the development of preventive agrochemicals for this important family of plant viruses.

Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles.,Byrne MJ, Steele JFC, Hesketh EL, Walden M, Thompson RF, Lomonossoff GP, Ranson NA Structure. 2019 Oct 8. pii: S0969-2126(19)30316-8. doi:, 10.1016/j.str.2019.09.010. PMID:31611039^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Byrne MJ, Steele JFC, Hesketh EL, Walden M, Thompson RF, Lomonossoff GP, Ranson NA. Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles. Structure. 2019 Oct 8. pii: S0969-2126(19)30316-8. doi:, 10.1016/j.str.2019.09.010. PMID:31611039 doi:http://dx.doi.org/10.1016/j.str.2019.09.010
↑ Adams MC, Schiltz CJ, Heck ML, Chappie JS. Crystal structure of the potato leafroll virus coat protein and implications for viral assembly. J Struct Biol. 2022 Mar;214(1):107811. PMID:34813955 doi:10.1016/j.jsb.2021.107811
↑ Byrne MJ, Steele JFC, Hesketh EL, Walden M, Thompson RF, Lomonossoff GP, Ranson NA. Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles. Structure. 2019 Oct 8. pii: S0969-2126(19)30316-8. doi:, 10.1016/j.str.2019.09.010. PMID:31611039 doi:http://dx.doi.org/10.1016/j.str.2019.09.010

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6sco"

Categories: Large Structures | Potato leafroll virus | Byrne MJ | Ranson NA

@@ Line 3: / Line 3: @@
 <SX load='6sco' size='340' side='right' viewer='molstar' caption='[[6sco]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6sco]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Plrv Plrv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SCO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SCO FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6sco]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Potato_leafroll_virus Potato leafroll virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SCO FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sco OCA], [http://pdbe.org/6sco PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sco RCSB], [http://www.ebi.ac.uk/pdbsum/6sco PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sco ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sco OCA], [https://pdbe.org/6sco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sco RCSB], [https://www.ebi.ac.uk/pdbsum/6sco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sco ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/CAPSD_PLRV1 CAPSD_PLRV1] Major capsid protein that self-assembles to form an icosahedral capsid with a T=3 symmetry, about 23 nm in diameter, and consisting of 180 capsid proteins monomers (PubMed:31611039, PubMed:34813955). Most of the 180 monomers are the major capsid protein, but a small percentage contain the minor capsid protein, which has a long C-terminal extension (PubMed:31611039).<ref>PMID:31611039</ref> <ref>PMID:34813955</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 18: @@
 </div>
 <div class="pdbe-citations 6sco" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 == References ==
 <references/>
@@ Line 20: / Line 26: @@
 </SX>
 [[Category: Large Structures]]
-[[Category: Plrv]]
+[[Category: Potato leafroll virus]]
-[[Category: Byrne, M J]]
+[[Category: Byrne MJ]]
-[[Category: Ranson, N A]]
+[[Category: Ranson NA]]
-[[Category: Capsid]]
-[[Category: Luteovirus]]
-[[Category: Polerovirus]]
-[[Category: Virus like particle]]
-[[Category: Vlp]]

6sco

From Proteopedia

Current revision

Cryo-EM Structure of Potato Leaf Roll Virus VLP

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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