6v6b

From Proteopedia

(Difference between revisions)

Current revision

Structures of GCP2 and GCP3 in the native human gamma-tubulin ring complex

6v6b, resolution 3.80Å

Structural highlights

6v6b is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.8Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GCP3_HUMAN Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.

Publication Abstract from PubMed

The gamma-tubulin ring complex (gamma-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we present a cryo-EM reconstruction of the native human gamma-TuRC at approximately 3.8 A resolution, revealing an asymmetric, cone-shaped structure. Pseudo-atomic models indicate that GCP4, GCP5, and GCP6 form distinct Y-shaped assemblies that structurally mimic GCP2/GCP3 subcomplexes distal to the gamma-TuRC "seam." We also identify an unanticipated structural bridge that includes an actin-like protein and spans the gamma-TuRC lumen. Despite its asymmetric architecture, the gamma-TuRC arranges gamma-tubulins into a helical geometry poised to nucleate microtubules. Diversity in the gamma-TuRC subunits introduces large (>100,000 A(2)) surfaces in the complex that allow for interactions with different regulatory factors. The observed compositional complexity of the gamma-TuRC could self-regulate its assembly into a cone-shaped structure to control microtubule formation across diverse contexts, e.g., within biological condensates or alongside existing filaments.

Asymmetric Molecular Architecture of the Human gamma-Tubulin Ring Complex.,Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM Cell. 2019 Dec 13. pii: S0092-8674(19)31369-8. doi: 10.1016/j.cell.2019.12.007. PMID:31862189^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM. Asymmetric Molecular Architecture of the Human gamma-Tubulin Ring Complex. Cell. 2019 Dec 13. pii: S0092-8674(19)31369-8. doi: 10.1016/j.cell.2019.12.007. PMID:31862189 doi:http://dx.doi.org/10.1016/j.cell.2019.12.007

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6v6b"

@@ Line 3: / Line 3: @@
 <SX load='6v6b' size='340' side='right' viewer='molstar' caption='[[6v6b]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6v6b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6V6B FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6v6b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V6B FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6v6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6b OCA], [http://pdbe.org/6v6b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v6b RCSB], [http://www.ebi.ac.uk/pdbsum/6v6b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6b ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6b OCA], [https://pdbe.org/6v6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v6b RCSB], [https://www.ebi.ac.uk/pdbsum/6v6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6b ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GCP3_HUMAN GCP3_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. [[http://www.uniprot.org/uniprot/GCP2_HUMAN GCP2_HUMAN]] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
+[https://www.uniprot.org/uniprot/GCP3_HUMAN GCP3_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 18: @@
 </div>
 <div class="pdbe-citations 6v6b" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tubulin 3D Structures|Tubulin 3D Structures]]
 == References ==
 <references/>
@@ Line 23: / Line 27: @@
 [[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Chait, B T]]
+[[Category: Chait BT]]
-[[Category: Kapoor, T M]]
+[[Category: Kapoor TM]]
-[[Category: Molloy, K R]]
+[[Category: Molloy KR]]
-[[Category: Ti, S]]
+[[Category: Ti S]]
-[[Category: Urnavicius, L]]
+[[Category: Urnavicius L]]
-[[Category: Wieczorek, M]]
+[[Category: Wieczorek M]]
-[[Category: G-turc]]
-[[Category: Gamma-tubulin ring complex]]
-[[Category: Gamma-tusc]]
-[[Category: Gcp]]
-[[Category: Gcp2]]
-[[Category: Gcp3]]
-[[Category: Gturc]]
-[[Category: Gtusc]]
-[[Category: Microtubule]]
-[[Category: Microtubule nucleation]]
-[[Category: Single particle cryo-em structure]]
-[[Category: Structural protein]]

6v6b

From Proteopedia

Current revision

Structures of GCP2 and GCP3 in the native human gamma-tubulin ring complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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