TRNA methyltransferase
From Proteopedia
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<StructureSection load='' size='350' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene='55/553109/Cv/1'> | <StructureSection load='' size='350' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry [[1uak]])' scene='55/553109/Cv/1'> | ||
| + | __TOC__ | ||
== Function == | == Function == | ||
| - | '''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction. | + | '''tRNA methyltransferase''' ('''Trm''') methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction. |
For details on Trm to N2 of guanine 6 see [[TRNA:m2G6 methyltransferase TrmN/Trm14]]. | For details on Trm to N2 of guanine 6 see [[TRNA:m2G6 methyltransferase TrmN/Trm14]]. | ||
| + | |||
| + | *'''Dual specificity tRNA methyltransferase Rlmn''' is an ''E. coli'' methyltransferase which modifies both ribosomal 23S RNA and tRNA<ref>PMID:22891362</ref>. | ||
| + | *'''METTL6''' modifies cytidine 32<ref>PMId:32923617</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
Trm <scene name='55/553109/Cv/4'>active site</scene> is located at the cleft at the subunit interface<ref>PMID:12773376</ref>. Water molecules are shown as red spheres. | Trm <scene name='55/553109/Cv/4'>active site</scene> is located at the cleft at the subunit interface<ref>PMID:12773376</ref>. Water molecules are shown as red spheres. | ||
| - | </StructureSection> | ||
| - | |||
==3D structures of tRNA methyltransferase== | ==3D structures of tRNA methyltransferase== | ||
| + | [[tRNA methyltransferase 3D structures]] | ||
| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
| - | |||
| - | *'''tRNA guanine 9 N1 methyltransferase''' | ||
| - | |||
| - | **[[4jwg]] – fyTrm – fission yeast<br /> | ||
| - | **[[4jwf]], [[4jwh]] – fyTrm + SAH <br /> | ||
| - | **[[4jwj]] – yTrm + SAH – yeast<br /> | ||
| - | |||
| - | *'''tRNA guanine 37 N1 methyltransferase''' | ||
| - | |||
| - | **[[3knu]] – ApTrm – ''Anaplasma phagocytophilum'' <br /> | ||
| - | **[[1oy5]] – AaTrm – ''Aquifex aeolicus'' <br /> | ||
| - | **[[3ief]] – Trm – ''Bartonella henselae'' <br /> | ||
| - | **[[1uaj]] – HiTrm – ''Haemophilus influenzae''<br /> | ||
| - | **[[3ky7]] – Trm – ''Staphylococcus aureus''<br /> | ||
| - | **[[3quv]] – Trm – ''Mycobacterium abscessus''<br /> | ||
| - | **[[5hjj]], [[5yac]] – PaTrm – ''Pyrococcus abyssi''<br /> | ||
| - | |||
| - | *Complexes of tRNA guanine 37 N1 methyltransferase | ||
| - | |||
| - | **[[1uak]], [[4yvg]] – HiTrm + SAM <br /> | ||
| - | **[[1ual]], [[1uam]] – HiTrm + SAH <br /> | ||
| - | **[[3axz]] – HiTrm + adenosine<br /> | ||
| - | **[[4mcb]], [[4mcc]], [[4mcd]] – HiTrm + inhibitor<br /> | ||
| - | **[[4yvh]] – HiTrm + SFG <br /> | ||
| - | **[[4yvi]], [[4yvj]], [[4yvk]] – HiTrm + SFG + tRNA<br /> | ||
| - | **[[5d9f]], [[4ypw]], [[4ypx]], [[4ypy]], [[4ypz]], [[4yq0]], [[4yq1]], [[4yq2]], [[4yq3]], [[4yq4]], [[4yq5]], [[4yq6]], [[4yq7]], [[4yq8]], [[4yq9]], [[4yqa]], [[4yqb]], [[4yqc]], [[4yqg]], [[4yqi]], [[4yqj]], [[4yqk]], [[4yql]], [[4yqn]], [[4yqo]], [[4yqp]], [[4yqq]], [[4yqr]], [[4yqs]], [[4yqt]], [[4yqd]] – HiTrm + SAM competitive<br /> | ||
| - | **[[2yx1]] – MjTrm + SFG – ''Methanocalcococcus jannaschii''<br /> | ||
| - | **[[3ay0]] – MjTrm + adenosine<br /> | ||
| - | **[[4h3y]], [[4h3z]] – Trm + SAH – ''Burkholderia phymatum'' <br /> | ||
| - | **[[4ig6]] – ApTrm + SAH <br /> | ||
| - | **[[1p9p]] – EcTrm + SAH – ''Escherichia coli'' <br /> | ||
| - | **[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br /> | ||
| - | **[[5hr7]] – EcTrm + tRNA-Glu<br /> | ||
| - | **[[5hji]], [[5hjm]] – PaTrm + adenosine derivative<br /> | ||
| - | **[[5hjk]] – PaTrm + SAH <br /> | ||
| - | |||
| - | *'''tRNA guanine 46 N7 methyltransferase''' | ||
| - | |||
| - | **[[2vdu]], [[2vdv]] – yTrm <br /> | ||
| - | **[[1yzh]] – Trm – ''Streptococcus pneumoniae'' <br /> | ||
| - | **[[2fca]] – Trm – ''Bacillus subtilis'' <br /> | ||
| - | **[[3dxx]] – EcTrm <br /> | ||
| - | |||
| - | *Complexes of tRNA guanine 46 N7 methyltransferase | ||
| - | |||
| - | **[[3ckk]] – hTrm + SAM - human<br /> | ||
| - | **[[3dxy]] – EcTrm + SAM <br /> | ||
| - | **[[3dxz]] – EcTrm + SAH <br /> | ||
| - | |||
| - | *Trm to O2 of cytidine 32 | ||
| - | |||
| - | **[[4cnd]] – EcTrm (mutant)<br /> | ||
| - | **[[4xbo]] – EcTrm + SAH<br /> | ||
| - | **[[4cne]] – EcTrm (mutant) + SAH<br /> | ||
| - | |||
| - | *'''tRNA cytidine 34 O2 methyltransferase''' | ||
| - | |||
| - | **[[3e5y]] – BpTrm – ''Burkholderia pseudomalllei'' <br /> | ||
| - | **[[4jak]], [[4kdz]] – EcTrm <br /> | ||
| - | |||
| - | *Complexes of tRNA cytidine 34 O2 methyltransferase | ||
| - | |||
| - | **[[4kgn]] – BpTrm + SAH <br /> | ||
| - | **[[3n4k]], [[3n4j]] – Trm + SAH – ''Yersinia pestis'' <br /> | ||
| - | **[[1mxi]], [[1im8]] – HiTrm + SAH <br /> | ||
| - | **[[4jal]] – EcTrm + SAH<br /> | ||
| - | |||
| - | *'''tRNA guanine 6 N2 methyltransferase''' | ||
| - | |||
| - | **[[3tm5]] – PfTrm + SFG – ''Pyrococcvus furiosus''<br /> | ||
| - | **[[3tlj]] – PfTrm + SAH<br /> | ||
| - | **[[3tm4]] – PfTrm + SAM<br /> | ||
| - | |||
| - | *Trm M2G/M22G10 | ||
| - | |||
| - | **[[5e71]] – TkTrm – ''Thermococcvus kodakarensis''<br /> | ||
| - | **[[5e72]] – TkTrm + SAM<br /> | ||
| - | |||
| - | *Trm to N1 of adenine 9 | ||
| - | |||
| - | **[[5a7t]], [[5a7z]] – SaTrm – ''Sulfolobus acidocaldarius''<br /> | ||
| - | **[[4cnf]] – SaTrm (mutant)<br /> | ||
| - | **[[5a7y]] – SaTrm + SAH<br /> | ||
| - | **[[4cng]] – SaTrm (mutant) + SAH<br /> | ||
| - | |||
| - | *'''tRNA adenine 58 N1 methyltransferase''' | ||
| - | |||
| - | **[[5ccb]], [[5cd1]] – hTrm + SAH + tRNA-Lys<br /> | ||
| - | **[[5ccx]] – hTrm + tRNA-Lys<br /> | ||
| - | **[[5eqj]] – yTrm <br /> | ||
| - | **[[5erg]] – yTrm + SAM<br /> | ||
| - | **[[2pwy]] – TtTrm – ''Thermus thermophilus''<br /> | ||
| - | **[[5c0o]], [[5c1i]] – TtTrm (mutant) + SAM<br /> | ||
| - | |||
| - | *Trm to uracil 34 | ||
| - | |||
| - | **[[4qnx]] – EcTrm <br /> | ||
| - | **[[4qnu]], [[4qnv]] – EcTrm + SAM derivative<br /> | ||
| - | |||
| - | *'''tRNA uracil 54 methyltransferase''' | ||
| - | |||
| - | **[[3bt7]] – yTrm + RNA<br /> | ||
| - | |||
| - | *Trm9-TRM112 | ||
| - | |||
| - | **[[5cm2]] – Trm – ''Yarrowia lipolytica''<br /> | ||
| - | |||
| - | *Trm dual specificity | ||
| - | |||
| - | **[[4pl1]], [[4pl2]] – EcTrm (mutant) + SAM<br /> | ||
| - | **[[6ems]], [[6emt]] – TkTrm <br /> | ||
| - | **[[6emu]] – TkTrm + SAM<br /> | ||
| - | **[[6emv]] –TkTrm + SAH<br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
| + | </StructureSection> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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