TRNA methyltransferase

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Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine (PDB entry 1uak)

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1 Function
2 Structural highlights
3 3D structures of tRNA methyltransferase
4 References

Function

tRNA methyltransferase (Trm) methylates specific positions on tRNA. tRNA methylation is a post-transcription modification. The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)^[1]. S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.

For details on Trm to N2 of guanine 6 see TRNA:m2G6 methyltransferase TrmN/Trm14.

Dual specificity tRNA methyltransferase Rlmn is an E. coli methyltransferase which modifies both ribosomal 23S RNA and tRNA^[2].
METTL6 modifies cytidine 32^[3].

Structural highlights

Trm is located at the cleft at the subunit interface^[4]. Water molecules are shown as red spheres.

3D structures of tRNA methyltransferase

tRNA methyltransferase 3D structures

References

↑ Swinehart WE, Jackman JE. Diversity in mechanism and function of tRNA methyltransferases. RNA Biol. 2015;12(4):398-411. doi: 10.1080/15476286.2015.1008358. PMID:25626150 doi:http://dx.doi.org/10.1080/15476286.2015.1008358
↑ Benitez-Paez A, Villarroya M, Armengod ME. The Escherichia coli RlmN methyltransferase is a dual-specificity enzyme that modifies both rRNA and tRNA and controls translational accuracy. RNA. 2012 Oct;18(10):1783-95. doi: 10.1261/rna.033266.112. Epub 2012 Aug 13. PMID:22891362 doi:http://dx.doi.org/10.1261/rna.033266.112
↑ Ignatova VV, Kaiser S, Ho JSY, Bing X, Stolz P, Tan YX, Lee CL, Gay FPH, Lastres PR, Gerlini R, Rathkolb B, Aguilar-Pimentel A, Sanz-Moreno A, Klein-Rodewald T, Calzada-Wack J, Ibragimov E, Valenta M, Lukauskas S, Pavesi A, Marschall S, Leuchtenberger S, Fuchs H, Gailus-Durner V, de Angelis MH, Bultmann S, Rando OJ, Guccione E, Kellner SM, Schneider R. METTL6 is a tRNA m(3)C methyltransferase that regulates pluripotency and tumor cell growth. Sci Adv. 2020 Aug 26;6(35):eaaz4551. PMID:32923617 doi:10.1126/sciadv.aaz4551
↑ Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK. Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition. EMBO J. 2003 Jun 2;22(11):2593-603. PMID:12773376 doi:10.1093/emboj/cdg269

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

Retrieved from "http://52.214.119.220/wiki/index.php/TRNA_methyltransferase"

Category: Topic Page

@@ Line 1: / Line 1: @@
 <StructureSection load='' size='350' side='right' caption='Structure of tRNA methyltransferase for N1 of guanine 37 complex with S-adenosylhomocysteine  (PDB entry [[1uak]])' scene='55/553109/Cv/1'>
+__TOC__
 == Function ==
-'''tRNA methyltransferase''' (Trm) methylates specific positions on tRNA.  tRNA methylation is a post-transcription modification.  The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>.  S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.
+'''tRNA methyltransferase''' ('''Trm''') methylates specific positions on tRNA.  tRNA methylation is a post-transcription modification.  The methyl donor is S-adenosylmethionine (SAM) and the product is the methylated tRNA and S-adenosylhomocysteine (SAH)<ref>PMID:25626150</ref>.  S-adenosylornithine or sinefugin (SFG) is an inhibitor of the reaction.
 For details on Trm to N2 of guanine 6 see [[TRNA:m2G6 methyltransferase TrmN/Trm14]].
+*'''Dual specificity tRNA methyltransferase Rlmn''' is an ''E. coli'' methyltransferase which modifies both ribosomal 23S RNA and tRNA<ref>PMID:22891362</ref>.
+*'''METTL6'''  modifies cytidine 32<ref>PMId:32923617</ref>.
 == Structural highlights ==
@@ Line 10: / Line 14: @@
 [[tRNA methyltransferase 3D structures]]
-</StructureSection>
-==3D structures of tRNA methyltransferase==
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
-{{#tree:id=OrganizedByTopic|openlevels=0|
-*'''tRNA guanine 9 N1 methyltransferase'''
-**[[4jwg]] – fyTrm – fission yeast<br />
-**[[4jwf]], [[4jwh]] – fyTrm + SAH <br />
-**[[4jwj]] – yTrm + SAH – yeast<br />
-*'''tRNA guanine 37 N1 methyltransferase'''
-**[[3knu]], [[6uev]] – ApTrm – ''Anaplasma phagocytophilum'' <br />
-**[[1oy5]] – AaTrm – ''Aquifex aeolicus'' <br />
-**[[3ief]] – Trm – ''Bartonella henselae'' <br />
-**[[1uaj]] – HiTrm – ''Haemophilus influenzae''<br />
-**[[3ky7]] – Trm – ''Staphylococcus aureus''<br />
-**[[3quv]], [[6nvr]] – MaTrm – ''Mycobacterium abscessus''<br />
-**[[5hjj]], [[5yac]] – PabTrm – ''Pyrococcus abyssi''<br />
-**[[6jki]] – PaTrm – ''Pseudomonas aeruginosa''<br />
-**[[5zhi]] – MtTrm – ''Mycobacterium tuberculosis''<br />
-*Complexes of tRNA guanine 37 N1 methyltransferase
-**[[1uak]], [[4yvg]] – HiTrm + SAM <br />
-**[[1ual]], [[1uam]] – HiTrm + SAH <br />
-**[[3axz]] – HiTrm + adenosine<br />
-**[[4mcb]], [[4mcc]], [[4mcd]] – HiTrm + inhibitor<br />
-**[[4yvh]] – HiTrm + SFG <br />
-**[[4yvi]], [[4yvj]], [[4yvk]] – HiTrm + SFG + tRNA<br />
-**[[5d9f]], [[4ypw]], [[4ypx]], [[4ypy]], [[4ypz]], [[4yq0]], [[4yq1]], [[4yq2]], [[4yq3]], [[4yq4]], [[4yq5]], [[4yq6]], [[4yq7]], [[4yq8]], [[4yq9]], [[4yqa]], [[4yqb]], [[4yqc]], [[4yqg]], [[4yqi]], [[4yqj]], [[4yqk]], [[4yql]], [[4yqn]], [[4yqo]], [[4yqp]], [[4yqq]], [[4yqr]], [[4yqs]], [[4yqt]], [[4yqd]] – HiTrm + SAM competitive<br />
-**[[2yx1]] – MjTrm + SFG – ''Methanocalcococcus jannaschii''<br />
-**[[3ay0]] – MjTrm + adenosine<br />
-**[[4h3y]], [[4h3z]] – Trm + SAH – ''Burkholderia phymatum'' <br />
-**[[4ig6]] – ApTrm + SAH <br />
-**[[1p9p]] – EcTrm + SAH – ''Escherichia coli'' <br />
-**[[4gek]], [[4iwn]] – EcTrm + SAM derivative<br />
-**[[5hr7]] – EcTrm + tRNA-Glu<br />
-**[[5hji]], [[5hjm]] – PabTrm + adenosine derivative<br />
-**[[5hjk]] – PabTrm + SAH <br />
-**[[6qo2]], [[6qo3]], [[6qo4]], [[6qo6]], [[6qoa]], [[6qoc]], [[6qod]], [[6qoe]], [[6qof]], [[6qog]], [[6qoh]], [[6qoi]], [[6qoj]], [[6qok]], [[6qol]], [[6qom]], [[6qon]], [[6qoo]], [[6qop]], [[6qoq]], [[6qor]], [[6qos]], [[6qot]], [[6qou]], [[6qov]], [[6qow]], [[6qox]], [[6qqq]], [[6qqr]], [[6qqt]], [[6qqu]], [[6qqv]], [[6qqw]], [[6qqz]], [[6qr0]], [[6qr1]], [[6qr2]], [[6qr3]], [[6qr4]], [[6qr7]], [[6qr9]], [[6qra]], [[6qrc]], [[6qrd]], [[6qre]], [[6qrf]], [[6qrg]] – MaTrm + inhibitor<br />
-**[[6nw6]] – MaTrm + SAM <br />
-**[[6nw7]] – MaTrm + SAH <br />
-**[[6joe]], [[6jof]], [[5zhm]], [[5zhn]], [[6afk]] – PaTrm + inhibitor<br />
-**[[6wyq]] – PaTrm + SAM <br />
-**[[6wyr]] – PaTrm + SFG <br />
-**[[5zhj]] – MtTrm + SAH <br />
-**[[5zhk]], [[5zhl]] – MtTrm + inhibitor<br />
-**[[5wt1]] – PabTrm + tRNA + SAH <br />
-**[[5wt3]] – PabTrm + tRNA + methylthioadenosine<br />
-*'''tRNA guanine 46 N7 methyltransferase'''
-**[[2vdu]], [[2vdv]] – yTrm  <br />
-**[[1yzh]] – Trm – ''Streptococcus pneumoniae'' <br />
-**[[2fca]] – BsTrm – ''Bacillus subtilis'' <br />
-**[[3dxx]] – EcTrm  <br />
-*Complexes of tRNA guanine 46 N7 methyltransferase
-**[[3ckk]] – hTrm + SAM - human<br />
-**[[3dxy]] – EcTrm + SAM <br />
-**[[3dxz]] – EcTrm + SAH <br />
-*Trm to O2 of cytidine 32
-**[[4cnd]] – EcTrm (mutant)<br />
-**[[4xbo]] – EcTrm + SAH<br />
-**[[4cne]] – EcTrm (mutant) + SAH<br />
-*'''tRNA cytidine 34 O2 methyltransferase'''
-**[[6jp6]] – yTrm <br />
-**[[3e5y]] – BpTrm – ''Burkholderia pseudomalllei'' <br />
-**[[4jak]], [[4kdz]] – EcTrm <br />
-**[[6qkv]] – PaTrm <br />
-**[[6ahw]] – HiTrm <br />
-*Complexes of tRNA cytidine 34 O2 methyltransferase
-**[[6jpl]] – yTrm + SAM  <br />
-**[[4kgn]] – BpTrm + SAH  <br />
-**[[3n4k]], [[3n4j]] – Trm + SAH – ''Yersinia pestis'' <br />
-**[[1mxi]], [[1im8]] – HiTrm + SAH  <br />
-**[[4jal]] – EcTrm + SAH<br />
-Trm to C5 of cytidine 38
-**[[6fdf]] – fyTrm + SAH  <br />
-Trm to O2 of cytidine/uridine
-**[[5gra]] – Trm – ''Zymomonas mobilis'' <br />
-**[[5gmc]], [[5gmb]] – PaTrm <b />
-**[[5gm8]] – PaTrm + SFG<br />
-*'''tRNA guanine 6 N2 methyltransferase'''
-**[[3tm5]] – PfTrm + SFG – ''Pyrococcvus furiosus''<br />
-**[[3tlj]] – PfTrm + SAH<br />
-**[[3tm4]] – PfTrm + SAM<br />
-*Trm M2G/M22G10
-**[[5e71]] – TkTrm – ''Thermococcvus kodakarensis''<br />
-**[[5e72]] – TkTrm + SAM<br />
-*Trm to N1 of adenine 9
-**[[5a7t]], [[5a7z]] – SaTrm – ''Sulfolobus acidocaldarius''<br />
-**[[4cnf]] – SaTrm (mutant)<br />
-**[[5a7y]] – SaTrm + SAH<br />
-**[[4cng]] – SaTrm (mutant) + SAH<br />
-*Trm to N1 of adenine 22
-**[[6q56]] – BsTrm (mutant)<br />
-**[[6qe6]] – BsTrm + SAH<br />
-*'''tRNA adenine 58 N1 methyltransferase'''
-**[[5ccb]], [[5cd1]] – hTrm + SAH + tRNA-Lys<br />
-**[[5ccx]] – hTrm + tRNA-Lys<br />
-**[[5eqj]] – yTrm <br />
-**[[5erg]] – yTrm + SAM<br />
-**[[2pwy]] – TtTrm – ''Thermus thermophilus''<br />
-**[[5c0o]], [[5c1i]] – TtTrm (mutant) + SAM<br />
-*Trm to uracil 34
-**[[4qnx]] – EcTrm <br />
-**[[4qnu]], [[4qnv]] – EcTrm + SAM derivative<br />
-*'''tRNA uracil 54 methyltransferase'''
-**[[3bt7]] – yTrm + RNA<br />
-*Trm9-TRM112
-**[[5cm2]] – Trm – ''Yarrowia lipolytica''<br />
-*Trm dual specificity
-**[[4pl1]], [[4pl2]] – EcTrm (mutant) + SAM<br />
-**[[6ems]], [[6emt]] – TkTrm <br />
-**[[6emu]] – TkTrm + SAM<br />
-**[[6emv]] –TkTrm + SAH<br />
-}}
 == References ==
 <references/>
+</StructureSection>
 [[Category:Topic Page]]

TRNA methyltransferase

From Proteopedia

Current revision

Contents

Function

Structural highlights

3D structures of tRNA methyltransferase

References

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