1hlb

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(New page: 200px<br /> <applet load="1hlb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hlb, resolution 2.5&Aring;" /> ''''''<br /> ==Overvie...)
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[[Image:1hlb.gif|left|200px]]<br />
 
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<applet load="1hlb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hlb, resolution 2.5&Aring;" />
 
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''''''<br />
 
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==Overview==
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==Structural analysis of monomeric hemichrome and dimeric cyanomet hemoglobins from Caudina arenicola==
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The X-ray structures of two hemoglobins (Hb) from the sea cucumber Caudina, arenicola (an echinoderm) have been determined: a low spin, hemichrome, monomeric Hb-C chain, and a cyanomet-liganded dimeric Hb-D chain. Attempts, to obtain crystal structures of the deoxy-liganded and hemichrome forms, from the same chain type have not been successful. In this work, the Hb-C, chain and Hb-D chain structures are compared, and differences observed in, tertiary structure related to the different ligand states for hemoglobin, chains from this organism. In addition to shifts of the distal histidine, and E helix, differences are noted in the position of the heme group, within the heme pocket, the hydrogen bonding of the heme group to the, protein, and the status of the D helix. These differences are important in, understanding the ligand-linked association states of these hemoglobins., The quaternary structure of the Hb-D homodimer is compared with those from, two other invertebrate hemoglobins from Scapharca inaequivalvis and, Urechis caupo, which also have subunit-subunit interactions that involve, the E and E' helices. The dimer interactions of the Caudina and Urechis, hemoglobins are quite dissimilar. However, the dimer interface observed in, cyanomet Hb-D is strikingly similar to that observed for the carbonmonoxy, hemoglobin dimer from the clam, Scapharca, yet many of the key amino acid, residues implicated in the cooperative mechanism of the Scapharca, hemoglobin are not conserved in the Caudina hemoglobins.
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<StructureSection load='1hlb' size='340' side='right'caption='[[1hlb]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hlb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Molpadia_arenicola Molpadia arenicola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HLB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HLB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hlb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hlb OCA], [https://pdbe.org/1hlb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hlb RCSB], [https://www.ebi.ac.uk/pdbsum/1hlb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hlb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLBC_MOLAR GLBC_MOLAR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/1hlb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hlb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1HLB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HLB OCA].
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural analysis of monomeric hemichrome and dimeric cyanomet hemoglobins from Caudina arenicola., Mitchell DT, Kitto GB, Hackert ML, J Mol Biol. 1995 Aug 18;251(3):421-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7650740 7650740]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Molpadia arenicola]]
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[[Category: Thermomicrobium roseum]]
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[[Category: Hackert ML]]
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[[Category: Hackert, M.L.]]
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[[Category: Mitchell DT]]
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[[Category: Mitchell, D.T.]]
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[[Category: HEM]]
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[[Category: oxygen transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 13:08:17 2007''
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Current revision

Structural analysis of monomeric hemichrome and dimeric cyanomet hemoglobins from Caudina arenicola

PDB ID 1hlb

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