6a4m

Structural highlights

Function

PUCL_BACSU Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.^{[1] [2] [3]}

See Also

• Urate oxidase 3D structures

References

1. ↑ Schultz AC, Nygaard P, Saxild HH. Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator. J Bacteriol. 2001 Jun;183(11):3293-302. doi: 10.1128/JB.183.11.3293-3302.2001. PMID:11344136 doi:http://dx.doi.org/10.1128/JB.183.11.3293-3302.2001
2. ↑ Kim K, Park J, Rhee S. Structural and functional basis for (S)-allantoin formation in the ureide pathway. J Biol Chem. 2007 Aug 10;282(32):23457-64. Epub 2007 Jun 13. PMID:17567580 doi:10.1074/jbc.M703211200
3. ↑ Pfrimer P, de Moraes LM, Galdino AS, Salles LP, Reis VC, De Marco JL, Prates MV, Bloch C Jr, Torres FA. Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli. J Biomed Biotechnol. 2010;2010:674908. doi: 10.1155/2010/674908. Epub 2010 Feb 4. PMID:20168977 doi:http://dx.doi.org/10.1155/2010/674908