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| | <StructureSection load='5bob' size='340' side='right'caption='[[5bob]], [[Resolution|resolution]] 1.50Å' scene=''> | | <StructureSection load='5bob' size='340' side='right'caption='[[5bob]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5bob]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Strsy Strsy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BOB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BOB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5bob]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_suis_05ZYH33 Streptococcus suis 05ZYH33]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BOB FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bob OCA], [https://pdbe.org/5bob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bob RCSB], [https://www.ebi.ac.uk/pdbsum/5bob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bob ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5boa|5boa]]</td></tr>
| + | |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSU05_0272 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=391295 STRSY])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5bob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bob OCA], [http://pdbe.org/5bob PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5bob RCSB], [http://www.ebi.ac.uk/pdbsum/5bob PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5bob ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A4VT01_STRSY A4VT01_STRSY] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strsy]] | + | [[Category: Streptococcus suis 05ZYH33]] |
| - | [[Category: Jiang, Y]] | + | [[Category: Jiang Y]] |
| - | [[Category: Yu, Y]] | + | [[Category: Yu Y]] |
| - | [[Category: Zhang, C]] | + | [[Category: Zhang C]] |
| - | [[Category: Beta sandwich core]]
| + | |
| - | [[Category: Translation]]
| + | |
| Structural highlights
Function
A4VT01_STRSY
Publication Abstract from PubMed
Fhb is a surface virulence protein from Streptococcus suis, which could aid bacterial evasion of host innate immune defense by recruiting complement regulator factor H to inactivate C3b deposited on bacterial surface in blood. Here we successfully expressed and purified the N terminal domain of Fhb (N-Fhb) and obtained crystals of the N-Fhb by sitting-drop vapor diffusion method with a resolution of 1.50 A. The crystals belong to space group C2 with unit cell parameters a = 127.1 A, b = 77.3 A, c = 131.6 A, alpha = 90 degrees , beta = 115.9 degrees , gamma = 90 degrees . The structure of N-Fhb was determined by SAD method and the core structure of N-Fhb is a beta sandwich. We speculated that binding of Fhb to human factor H may be mainly mediated by surface amino acids with negative charges.
Expression, purification, crystallization and structure determination of the N terminal domain of Fhb, a factor H binding protein from Streptococcus suis.,Zhang C, Yu Y, Yang M, Jiang Y Biochem Biophys Res Commun. 2015 Oct 23;466(3):413-7. doi:, 10.1016/j.bbrc.2015.09.040. Epub 2015 Sep 10. PMID:26365348[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhang C, Yu Y, Yang M, Jiang Y. Expression, purification, crystallization and structure determination of the N terminal domain of Fhb, a factor H binding protein from Streptococcus suis. Biochem Biophys Res Commun. 2015 Oct 23;466(3):413-7. doi:, 10.1016/j.bbrc.2015.09.040. Epub 2015 Sep 10. PMID:26365348 doi:http://dx.doi.org/10.1016/j.bbrc.2015.09.040
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