1jf3

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(New page: 200px<br /> <applet load="1jf3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jf3, resolution 1.4&Aring;" /> '''Crystal Structure Of...)
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[[Image:1jf3.gif|left|200px]]<br />
 
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<applet load="1jf3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jf3, resolution 1.4&Aring;" />
 
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'''Crystal Structure Of Component III Glycera Dibranchiata Monomeric Hemoglobin'''<br />
 
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==Overview==
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==Crystal Structure Of Component III Glycera Dibranchiata Monomeric Hemoglobin==
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Erythrocytes of the marine annelid, Glycera dibranchiata, contain a, mixture of monomeric and polymeric hemoglobins. There are three major, monomer hemoglobin components, II, III, IV (also called GMH2, 3, and 4), that have been highly purified and well characterized. We have now, crystallized GMH3 and GMH4 and determined their structures to 1.4-1.8 A, resolution. The structures were determined for these two monomer, hemoglobins in the oxidized (Fe3+, ferric, or met-) forms in both the, unligated and cyanide-ligated states. This work differs from two, published, refined structures of a Glycera dibranchiata monomer, hemoglobin, which has a sequence that is substantially different from any, bona fide major monomer hemoglobins (GMH2, 3, or 4). The high-resolution, crystal structures (presented here) and the previous NMR structure of, CO-ligated GMH4, provide a basis for interpreting structure/function, details of the monomer hemoglobins. These details include: (1) the strong, correlation between temperature factor and NMR dynamics for respective, protein forms; (2) the unique nature of the HisE7Leu primary sequence, substitutions in GMH3 and GMH4 and their impact on cyanide ion binding, kinetics; (3) the LeuB10Phe difference between GMH3 and GMH4 and its, impact on ligand binding; and (4) elucidation of changes in the structural, details of the distal and proximal heme pockets upon cyanide binding.
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<StructureSection load='1jf3' size='340' side='right'caption='[[1jf3]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jf3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycera_dibranchiata Glycera dibranchiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JF3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jf3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jf3 OCA], [https://pdbe.org/1jf3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jf3 RCSB], [https://www.ebi.ac.uk/pdbsum/1jf3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jf3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLB1_GLYDI GLB1_GLYDI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jf/1jf3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jf3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JF3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Glycera_dibranchiata Glycera dibranchiata] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JF3 OCA].
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structures of unligated and CN-ligated Glycera dibranchiata monomer ferric hemoglobin components III and IV., Park HJ, Yang C, Treff N, Satterlee JD, Kang C, Proteins. 2002 Oct 1;49(1):49-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12211015 12211015]
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[[Category: Glycera dibranchiata]]
[[Category: Glycera dibranchiata]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kang, C.H.]]
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[[Category: Kang CH]]
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[[Category: Park, H.J.]]
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[[Category: Park HJ]]
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[[Category: Satterlee, J.D.]]
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[[Category: Satterlee JD]]
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[[Category: Treff, N.]]
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[[Category: Treff N]]
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[[Category: Yang, C.]]
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[[Category: Yang C]]
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[[Category: HEM]]
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[[Category: glycera]]
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[[Category: monomer hemoglobin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 13:10:14 2007''
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Current revision

Crystal Structure Of Component III Glycera Dibranchiata Monomeric Hemoglobin

PDB ID 1jf3

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