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| | <StructureSection load='4p5i' size='340' side='right'caption='[[4p5i]], [[Resolution|resolution]] 2.25Å' scene=''> | | <StructureSection load='4p5i' size='340' side='right'caption='[[4p5i]], [[Resolution|resolution]] 2.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4p5i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Contagious_pustular_dermatitis_virus Contagious pustular dermatitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P5I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P5I FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4p5i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Orf_virus Orf virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P5I FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ffk|2ffk]], [[2grk|2grk]], [[2vga|2vga]], [[1cq3|1cq3]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p5i OCA], [http://pdbe.org/4p5i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4p5i RCSB], [http://www.ebi.ac.uk/pdbsum/4p5i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4p5i ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p5i OCA], [https://pdbe.org/4p5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p5i RCSB], [https://www.ebi.ac.uk/pdbsum/4p5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p5i ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q2F862_ORFV Q2F862_ORFV] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Contagious pustular dermatitis virus]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Counago, R M]] | + | [[Category: Orf virus]] |
| - | [[Category: Krause, K L]] | + | [[Category: Counago RM]] |
| - | [[Category: Cytokine]] | + | [[Category: Krause KL]] |
| - | [[Category: Host chemokine]]
| + | |
| - | [[Category: Secreted]]
| + | |
| - | [[Category: Viral protein]]
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| Structural highlights
Function
Q2F862_ORFV
Publication Abstract from PubMed
The chemokine binding protein (CKBP) from orf virus (ORFV) binds with high affinity to chemokines from three classes, C, CC, and CXC, making it unique among poxvirus CKBPs described to date. We present its crystal structure alone and in complex with three CC chemokines, CCL2, CCL3, and CCL7. ORFV CKBP possesses a beta-sandwich fold that is electrostatically and sterically complementary to its binding partners. Chemokines bind primarily through interactions involving the N-terminal loop and a hydrophobic recess on the ORFV CKBP beta-sheet II surface, and largely polar interactions between the chemokine 20s loop and a negatively charged surface groove located at one end of the CKBP beta-sheet II surface. ORFV CKBP interacts with leukocyte receptor and glycosaminoglycan binding sites found on the surface of bound chemokines. SEC-MALLS and chromatographic evidence is presented supporting that ORFV CKBP is a dimer in solution over a broad range of protein concentrations.
Structures of Orf Virus Chemokine Binding Protein in Complex with Host Chemokines Reveal Clues to Broad Binding Specificity.,Counago RM, Knapp KM, Nakatani Y, Fleming SB, Corbett M, Wise LM, Mercer AA, Krause KL Structure. 2015 Jun 10. pii: S0969-2126(15)00192-6. doi:, 10.1016/j.str.2015.04.023. PMID:26095031[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Counago RM, Knapp KM, Nakatani Y, Fleming SB, Corbett M, Wise LM, Mercer AA, Krause KL. Structures of Orf Virus Chemokine Binding Protein in Complex with Host Chemokines Reveal Clues to Broad Binding Specificity. Structure. 2015 Jun 10. pii: S0969-2126(15)00192-6. doi:, 10.1016/j.str.2015.04.023. PMID:26095031 doi:http://dx.doi.org/10.1016/j.str.2015.04.023
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