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5c5v

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<StructureSection load='5c5v' size='340' side='right'caption='[[5c5v]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
<StructureSection load='5c5v' size='340' side='right'caption='[[5c5v]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5c5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tryb2 Tryb2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C5V FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5c5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei_TREU927 Trypanosoma brucei brucei TREU927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C5V FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PN:IMIDODIPHOSPHORIC+ACID'>2PN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tb11.02.4910 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=185431 TRYB2])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PN:IMIDODIPHOSPHORIC+ACID'>2PN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c5v OCA], [http://pdbe.org/5c5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c5v RCSB], [http://www.ebi.ac.uk/pdbsum/5c5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c5v ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c5v OCA], [https://pdbe.org/5c5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c5v RCSB], [https://www.ebi.ac.uk/pdbsum/5c5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c5v ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q384W5_TRYB2 Q384W5_TRYB2]
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Trypanosoma brucei (T. brucei) is responsible for the fatal human disease called African trypanosomiasis or sleeping sickness. The causative parasite Trypanosoma encodes soluble versions of inorganic pyrophosphatases (PPase), also called vacuolar soluble proteins (VSPs), which are localized to its acidocalcisomes. Latter are acidic membrane enclosed organelles rich in polyphosphate chains and divalent cations whose significance in these parasites remains unclear. We here report the crystal structure of T. brucei brucei acidocalcisomal PPases in a ternary complex with Mg2+ and imidodiphosphate. The crystal structure reveals a novel structural architecture distinct from known class I PPases in its tetrameric oligomeric state in which a fused EF-hand domain arranges around the catalytic PPase domain. This unprecedented assembly evident from TbbVSP1 crystal structure is further confirmed by small angle X-ray scattering (SAXS) and electron microscopy data. Solution scattering data suggests structural flexibility in EF-hand domains indicative of conformational plasticity within TbbVSP1.
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Structural and Functional highlights of Vacuolar Soluble Protein 1 from pathogen T. brucei. brucei.,Jamwal A, Round AR, Bannwarth L, Bryan CV, Belrahli H, Yogavel M, Sharma A J Biol Chem. 2015 Oct 22. pii: jbc.M115.674176. PMID:26494625<ref>PMID:26494625</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5c5v" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Tryb2]]
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[[Category: Trypanosoma brucei brucei TREU927]]
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[[Category: Jamwal, A]]
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[[Category: Jamwal A]]
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[[Category: Sharma, A]]
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[[Category: Sharma A]]
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[[Category: Yogavel, M]]
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[[Category: Yogavel M]]
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[[Category: Ca binding protein]]
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[[Category: Ef-hand]]
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[[Category: Hydrolase]]
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[[Category: Ppase domain]]
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Current revision

Recombinant Inorganic Pyrophosphatase from T brucei brucei

PDB ID 5c5v

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