6m77
From Proteopedia
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(New page: '''Unreleased structure''' The entry 6m77 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures) |
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- | '''Unreleased structure''' | ||
- | + | ==GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine== | |
+ | <StructureSection load='6m77' size='340' side='right'caption='[[6m77]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[6m77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_ATCC_10100 Enterococcus faecalis ATCC 10100]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M77 FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m77 OCA], [https://pdbe.org/6m77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m77 RCSB], [https://www.ebi.ac.uk/pdbsum/6m77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m77 ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/A0A125W693_ENTFL A0A125W693_ENTFL] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Glycoside hydrolases catalyze the hydrolysis of glycosidic linkages in carbohydrates. The glycoside hydrolase family 31 (GH31) contains alpha-glucosidase, alpha-xylosidase, alpha-galactosidase, and alpha-transglycosylase. Recent work has expanded the diversity of substrate specificity of GH31 enzymes, and alpha-N-acetylgalactosaminidases (alphaGalNAcases) belonging to GH31 have been identified in human gut bacteria. Here, we determined the first crystal structure of a truncated form of GH31 alphaGalNAcase from the human gut bacterium Enterococcus faecalis. The enzyme has a similar fold to other reported GH31 enzymes and an additional fibronectin type 3-like domain. Additionally, the structure in complex with N-acetylgalactosamine reveals that conformations of the active site residues, including its catalytic nucleophile, change to recognize the ligand. Our structural analysis provides insight into the substrate recognition and catalytic mechanism of GH31 alphaGalNAcases. | ||
- | + | Crystal structure of the Enterococcus faecalis alpha-N-acetylgalactosaminidase, a member of the glycoside hydrolase family 31.,Miyazaki T, Park EY FEBS Lett. 2020 May 4. doi: 10.1002/1873-3468.13804. PMID:32367553<ref>PMID:32367553</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
+ | <div class="pdbe-citations 6m77" style="background-color:#fffaf0;"></div> | ||
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </StructureSection> | ||
+ | [[Category: Enterococcus faecalis ATCC 10100]] | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Miyazaki T]] |
Current revision
GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine
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