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2gas

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Crystal Structure of Isoflavone Reductase

Structural highlights

2gas is a 2 chain structure with sequence from Medicago sativa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IFR_MEDSA Reduces achiral isoflavones to chiral isoflavanones during the biosynthesis of chiral pterocarpan phytoalexins. The reduction product is a third isomer, which represents the penultimate intermediate in the synthesis of the phytoalexin (-)-medicarpin, the major phytoalexin in Alfalfa.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Isoflavonoids play important roles in plant defense and exhibit a range of mammalian health-promoting activities. Isoflavone reductase (IFR) specifically recognizes isoflavones and catalyzes a stereospecific NADPH-dependent reduction to (3R)-isoflavanone. The crystal structure of Medicago sativa IFR with deletion of residues 39-47 has been determined at 1.6A resolution. Structural analysis, molecular modeling and docking, and comparison with the structures of other NADPH-dependent enzymes, defined the putative binding sites for co-factor and substrate and potential key residues for enzyme activity and substrate specificity. Further mutagenesis has confirmed the role of Lys144 as a catalytic residue. This study provides a structural basis for understanding the enzymatic mechanism and substrate specificity of IFRs as well as the functions of IFR-like proteins.

Crystal structure of isoflavone reductase from alfalfa (Medicago sativa L.).,Wang X, He X, Lin J, Shao H, Chang Z, Dixon RA J Mol Biol. 2006 May 19;358(5):1341-52. Epub 2006 Mar 29. PMID:16600295^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang X, He X, Lin J, Shao H, Chang Z, Dixon RA. Crystal structure of isoflavone reductase from alfalfa (Medicago sativa L.). J Mol Biol. 2006 May 19;358(5):1341-52. Epub 2006 Mar 29. PMID:16600295 doi:10.1016/j.jmb.2006.03.022

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gas"

@@ Line 3: / Line 3: @@
 <StructureSection load='2gas' size='340' side='right'caption='[[2gas]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2gas]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Alfalfa Alfalfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2GAS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2gas]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GAS FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gas OCA], [http://pdbe.org/2gas PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2gas RCSB], [http://www.ebi.ac.uk/pdbsum/2gas PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2gas ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gas OCA], [https://pdbe.org/2gas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gas RCSB], [https://www.ebi.ac.uk/pdbsum/2gas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gas ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/IFR_MEDSA IFR_MEDSA]] Reduces achiral isoflavones to chiral isoflavanones during the biosynthesis of chiral pterocarpan phytoalexins. The reduction product is a third isomer, which represents the penultimate intermediate in the synthesis of the phytoalexin (-)-medicarpin, the major phytoalexin in Alfalfa.
+[https://www.uniprot.org/uniprot/IFR_MEDSA IFR_MEDSA] Reduces achiral isoflavones to chiral isoflavanones during the biosynthesis of chiral pterocarpan phytoalexins. The reduction product is a third isomer, which represents the penultimate intermediate in the synthesis of the phytoalexin (-)-medicarpin, the major phytoalexin in Alfalfa.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Alfalfa]]
 [[Category: Large Structures]]
-[[Category: Chang, Z]]
+[[Category: Medicago sativa]]
-[[Category: Dixon, R A]]
+[[Category: Chang Z]]
-[[Category: He, X]]
+[[Category: Dixon RA]]
-[[Category: Lin, J]]
+[[Category: He X]]
-[[Category: Shao, H]]
+[[Category: Lin J]]
-[[Category: Wang, X]]
+[[Category: Shao H]]
-[[Category: Nadph-dependent reductase]]
+[[Category: Wang X]]
-[[Category: Oxidoreductase]]

2gas

From Proteopedia

Current revision

Crystal Structure of Isoflavone Reductase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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