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| <StructureSection load='5c9p' size='340' side='right'caption='[[5c9p]], [[Resolution|resolution]] 1.75Å' scene=''> | | <StructureSection load='5c9p' size='340' side='right'caption='[[5c9p]], [[Resolution|resolution]] 1.75Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[5c9p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29999 Atcc 29999]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C9P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5c9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_luminescens Photorhabdus luminescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C9P FirstGlance]. <br> |
- | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> |
- | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5c9l|5c9l]], [[5c9o|5c9o]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
- | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">plu0732 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29488 ATCC 29999])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9p OCA], [https://pdbe.org/5c9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c9p RCSB], [https://www.ebi.ac.uk/pdbsum/5c9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c9p ProSAT]</span></td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9p OCA], [http://pdbe.org/5c9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c9p RCSB], [http://www.ebi.ac.uk/pdbsum/5c9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c9p ProSAT]</span></td></tr> | + | |
| </table> | | </table> |
| + | == Function == |
| + | [https://www.uniprot.org/uniprot/Q7N8J0_PHOLL Q7N8J0_PHOLL] |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
- | [[Category: Atcc 29999]] | |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: Demo, G]] | + | [[Category: Photorhabdus luminescens]] |
- | [[Category: Dobes, P]] | + | [[Category: Demo G]] |
- | [[Category: Hyrsl, P]] | + | [[Category: Dobes P]] |
- | [[Category: Kumar, A]] | + | [[Category: Hyrsl P]] |
- | [[Category: Sykorova, P]] | + | [[Category: Kumar A]] |
- | [[Category: Wimmerova, M]] | + | [[Category: Sykorova P]] |
- | [[Category: Fucose-specific]]
| + | [[Category: Wimmerova M]] |
- | [[Category: Lectin]]
| + | |
- | [[Category: Seven-bladed beta-propeller]]
| + | |
- | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
Q7N8J0_PHOLL
Publication Abstract from PubMed
Photorhabdus luminescens is known for its symbiosis with the entomopathogenic nematode Heterorhabditis bacteriophora and its pathogenicity towards insect larvae. A hypothetical protein from P. luminescens was identified, purified from the native source and characterized as an L-fucose-binding lectin, named PLL. Glycan array and biochemical characterization data revealed PLL to be specific towards L-fucose and the disaccharide glycan 3,6-O-Me2-Glcbeta1-4(2,3-O-Me2)Rhaalpha-O-(p-C6H4)-OCH2CH2NH2. PLL was discovered to be a homo-tetramer with an inter-subunit disulphide bridge. The crystal structures of native (ntPLL) and recombinant PLL (rPLL) revealed a seven-bladed beta-propeller fold creating 7 putative fucose-binding sites per monomer. The crystal structure of the rPLL/L-fucose complex confirmed at least three sites being fucose-binding. Moreover, the crystal structures indicated that some of the other sites are masked either by the tetrameric nature of the lectin or by incorporation of the C-terminus of the lectin into one of these sites. PLL exhibited a binding ability to insect haemocytes and the cuticular surface of a nematode, Heterorhabditis bacteriophora.
A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Hepta-Bladed beta-Propeller Tetrameric Structure.,Kumar A, Sykorova P, Demo G, Dobes P, Hyrsl P, Wimmerova M J Biol Chem. 2016 Oct 7. pii: jbc.M115.693473. PMID:27758853[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kumar A, Sykorova P, Demo G, Dobes P, Hyrsl P, Wimmerova M. A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Hepta-Bladed beta-Propeller Tetrameric Structure. J Biol Chem. 2016 Oct 7. pii: jbc.M115.693473. PMID:27758853 doi:http://dx.doi.org/10.1074/jbc.M115.693473
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