1avg

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:28, 13 March 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1avg.gif|left|200px]]
 
-
<!--
+
==THROMBIN INHIBITOR FROM TRIATOMA PALLIDIPENNIS==
-
The line below this paragraph, containing "STRUCTURE_1avg", creates the "Structure Box" on the page.
+
<StructureSection load='1avg' size='340' side='right'caption='[[1avg]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1avg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Meccus_pallidipennis Meccus pallidipennis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AVG FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1avg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1avg OCA], [https://pdbe.org/1avg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1avg RCSB], [https://www.ebi.ac.uk/pdbsum/1avg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1avg ProSAT]</span></td></tr>
-
{{STRUCTURE_1avg| PDB=1avg | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/THRB_BOVIN THRB_BOVIN] Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/av/1avg_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1avg ConSurf].
 +
<div style="clear:both"></div>
-
'''THROMBIN INHIBITOR FROM TRIATOMA PALLIDIPENNIS'''
+
==See Also==
-
 
+
*[[Thrombin 3D Structures|Thrombin 3D Structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Triabin, a 142-residue protein from the saliva of the blood-sucking triatomine bug Triatoma pallidipennis, is a potent and selective thrombin inhibitor. Its stoichiometric complex with bovine alpha-thrombin was crystallized, and its crystal structure was solved by Patterson search methods and refined at 2.6-A resolution to an R value of 0.184. The analysis revealed that triabin is a compact one-domain molecule essentially consisting of an eight-stranded beta-barrel. The eight strands A to H are arranged in the order A-C-B-D-E-F-G-H, with the first four strands exhibiting a hitherto unobserved up-up-down-down topology. Except for the B-C inversion, the triabin fold exhibits the regular up-and-down topology of lipocalins. In contrast to the typical ligand-binding lipocalins, however, the triabin barrel encloses a hydrophobic core intersected by a unique salt-bridge cluster. Triabin interacts with thrombin exclusively via its fibrinogen-recognition exosite. Surprisingly, most of the interface interactions are hydrophobic. A prominent exception represents thrombin's Arg-77A side chain, which extends into a hydrophobic triabin pocket forming partially buried salt bridges with Glu-128 and Asp-135 of the inhibitor. The fully accessible active site of thrombin in this complex is in agreement with its retained hydrolytic activity toward small chromogenic substrates. Impairment of thrombin's fibrinogen converting activity or of its thrombomodulin-mediated protein C activation capacity upon triabin binding is explained by usage of overlapping interaction sites of fibrinogen, thrombomodulin, and triabin on thrombin. These data demonstrate that triabin inhibits thrombin via a novel and unique mechanism that might be of interest in the context of potential therapeutic applications.
+
-
 
+
-
==About this Structure==
+
-
1AVG is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Triatoma_pallidipennis Triatoma pallidipennis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVG OCA].
+
-
 
+
-
==Reference==
+
-
Structure of the thrombin complex with triabin, a lipocalin-like exosite-binding inhibitor derived from a triatomine bug., Fuentes-Prior P, Noeske-Jungblut C, Donner P, Schleuning WD, Huber R, Bode W, Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11845-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9342325 9342325]
+
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Thrombin]]
+
[[Category: Meccus pallidipennis]]
-
[[Category: Triatoma pallidipennis]]
+
[[Category: Bode W]]
-
[[Category: Bode, W.]]
+
[[Category: Fuentes-Prior P]]
-
[[Category: Fuentes-Prior, P.]]
+
[[Category: Huber R]]
-
[[Category: Huber, R.]]
+
-
[[Category: Bovine thrombin]]
+
-
[[Category: Thrombin inhibitor]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:44:36 2008''
+

Current revision

THROMBIN INHIBITOR FROM TRIATOMA PALLIDIPENNIS

PDB ID 1avg

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1avg"
Personal tools