1a99

From Proteopedia

(Difference between revisions)

Current revision

PUTRESCINE RECEPTOR (POTF) FROM E. COLI

Structural highlights

1a99 is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

POTF_ECOLI Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

PotF protein is a periplasmic substrate-binding protein of the putrescine transport system in Escherichia coli. We have determined the crystal structure of PotF protein in complex with the substrate at 2.3-A resolution. The PotF molecule has dimensions of 54 x 42 x 30 A and consists of two similar globular domains. The PotF structure is reminiscent of other periplasmic receptors with a highest structural homology to another polyamine-binding protein, PotD. Putrescine is tightly bound in the deep cleft between the two domains of PotF through 12 hydrogen bonds and 36 van der Waals interactions. The comparison of the PotF structure with that of PotD provides the insight into the differences in the specificity between the two proteins. The PotF structure, in combination with the mutational analysis, revealed the residues crucial for putrescine binding (Trp-37, Ser-85, Glu-185, Trp-244, Asp-247, and Asp-278) and the importance of water molecules for putrescine recognition.

Crystal structure and mutational analysis of the Escherichia coli putrescine receptor. Structural basis for substrate specificity.,Vassylyev DG, Tomitori H, Kashiwagi K, Morikawa K, Igarashi K J Biol Chem. 1998 Jul 10;273(28):17604-9. PMID:9651355^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vassylyev DG, Tomitori H, Kashiwagi K, Morikawa K, Igarashi K. Crystal structure and mutational analysis of the Escherichia coli putrescine receptor. Structural basis for substrate specificity. J Biol Chem. 1998 Jul 10;273(28):17604-9. PMID:9651355

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1a99"

@@ Line 3: / Line 3: @@
 <StructureSection load='1a99' size='340' side='right'caption='[[1a99]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1a99]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A99 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1A99 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1a99]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A99 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PUCPOTF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a99 OCA], [http://pdbe.org/1a99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1a99 RCSB], [http://www.ebi.ac.uk/pdbsum/1a99 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1a99 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a99 OCA], [https://pdbe.org/1a99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a99 RCSB], [https://www.ebi.ac.uk/pdbsum/1a99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a99 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/POTF_ECOLI POTF_ECOLI]] Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein.
+[https://www.uniprot.org/uniprot/POTF_ECOLI POTF_ECOLI] Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a9/1a99_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 33: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Igarashi, K]]
+[[Category: Igarashi K]]
-[[Category: Kashiwagi, K]]
+[[Category: Kashiwagi K]]
-[[Category: Morikawa, K]]
+[[Category: Morikawa K]]
-[[Category: Tomitori, H]]
+[[Category: Tomitori H]]
-[[Category: Vassylyev, D G]]
+[[Category: Vassylyev DG]]
-[[Category: Binding protein]]
-[[Category: Transport]]

1a99

From Proteopedia

Current revision

PUTRESCINE RECEPTOR (POTF) FROM E. COLI

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox