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| | <StructureSection load='5d21' size='340' side='right'caption='[[5d21]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='5d21' size='340' side='right'caption='[[5d21]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5d21]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D21 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5d21]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D21 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=56N:PHENYL+BETA-D-GALACTOPYRANOSIDE'>56N</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lecA, pa1L, ERS445055_02484, PA257_2995, PA8380_27900, PAE221_02397, PAMH19_6043, YQ19_15530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=56N:PHENYL+BETA-D-GALACTOPYRANOSIDE'>56N</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d21 OCA], [http://pdbe.org/5d21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d21 RCSB], [http://www.ebi.ac.uk/pdbsum/5d21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d21 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d21 OCA], [https://pdbe.org/5d21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d21 RCSB], [https://www.ebi.ac.uk/pdbsum/5d21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d21 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PA1L_PSEAE PA1L_PSEAE] D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bergmann, M]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: Darbre, T]] | + | [[Category: Bergmann M]] |
| - | [[Category: Jin, X]] | + | [[Category: Darbre T]] |
| - | [[Category: Michaud, G]] | + | [[Category: Jin X]] |
| - | [[Category: Reymond, J L]] | + | [[Category: Michaud G]] |
| - | [[Category: Stocker, A]] | + | [[Category: Reymond J-L]] |
| - | [[Category: Visini, R]] | + | [[Category: Stocker A]] |
| - | [[Category: Antimicrobial]]
| + | [[Category: Visini R]] |
| - | [[Category: Biofilm]]
| + | |
| - | [[Category: Lectin]]
| + | |
| - | [[Category: Multivalency]]
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| - | [[Category: Pseudomona]]
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| - | [[Category: Sugar binding protein]]
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| Structural highlights
Function
PA1L_PSEAE D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.
Publication Abstract from PubMed
The galactose specific lectin LecA partly mediates the formation of antibiotic resistant biofilms by Pseudomonas aeruginosa, an opportunistic pathogen causing lethal airways infections in immunocompromised and cystic fibrosis patients, suggesting that preventing LecA binding to natural saccharides might provide new opportunities for treatment. Here 8-fold (G3) and 16-fold (G4) galactosylated analogs of , a tetravalent G2 glycopeptide dendrimer LecA ligand and P. aeruginosa biofilm inhibitor, were obtained by convergent chloroacetyl thioether (ClAc) ligation between 4-fold or 8-fold chloroacetylated dendrimer cores and digalactosylated dendritic arms. Hemagglutination inhibition, isothermal titration calorimetry and biofilm inhibition assays showed that G3 dendrimers bind LecA slightly better than their parent G2 dendrimers and induce complete biofilm inhibition and dispersal of P. aeruginosa biofilms, while G4 dendrimers show reduced binding and no biofilm inhibition. A binding model accounting for the observed saturation of glycopeptide dendrimer galactosyl groups and LecA binding sites is proposed based on the crystal structure of a G3 dendrimer LecA complex.
Multivalency effects on Pseudomonas aeruginosa biofilm inhibition and dispersal by glycopeptide dendrimers targeting lectin LecA.,Bergmann M, Michaud G, Visini R, Jin X, Gillon E, Stocker A, Imberty A, Darbre T, Reymond JL Org Biomol Chem. 2015 Sep 29. PMID:26416170[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bergmann M, Michaud G, Visini R, Jin X, Gillon E, Stocker A, Imberty A, Darbre T, Reymond JL. Multivalency effects on Pseudomonas aeruginosa biofilm inhibition and dispersal by glycopeptide dendrimers targeting lectin LecA. Org Biomol Chem. 2015 Sep 29. PMID:26416170 doi:http://dx.doi.org/10.1039/c5ob01682g
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