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7bti
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7bti is ON HOLD Authors: Kumari, A., Ragunath, V.K., Sirajuddin, M. Description: Phalloidin bound F-actin complex [[Category: Unreleased Structures...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Phalloidin bound F-actin complex== | |
| + | <StructureSection load='7bti' size='340' side='right'caption='[[7bti]], [[Resolution|resolution]] 3.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7bti]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BTI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BTI FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALO:ALLO-THREONINE'>ALO</scene>, <scene name='pdbligand=G5G:'>G5G</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bti OCA], [http://pdbe.org/7bti PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bti RCSB], [http://www.ebi.ac.uk/pdbsum/7bti PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bti ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/ACTS_CHICK ACTS_CHICK]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cellular studies of filamentous actin (F-actin) processes commonly utilize fluorescent versions of toxins, peptides, and proteins that bind actin. While the choice of these markers has been largely based on availability and ease, there is a severe dearth of structural data for an informed judgment in employing suitable F-actin markers for a particular requirement. Here, we describe the electron cryomicroscopy structures of phalloidin, lifeAct, and utrophin bound to F-actin, providing a comprehensive high-resolution structural comparison of widely used actin markers and their influence towards F-actin. Our results show that phalloidin binding does not induce specific conformational change and lifeAct specifically recognizes closed D-loop conformation, i.e., ADP-Pi or ADP states of F-actin. The structural models aided designing of minimal utrophin and a shorter lifeAct, which can be utilized as F-actin marker. Together, our study provides a structural perspective, where the binding sites of utrophin and lifeAct overlap with majority of actin-binding proteins and thus offering an invaluable resource for researchers in choosing appropriate actin markers and generating new marker variants. | ||
| - | + | Structural insights into actin filament recognition by commonly used cellular actin markers.,Kumari A, Kesarwani S, Javoor MG, Vinothkumar KR, Sirajuddin M EMBO J. 2020 Jul 15;39(14):e104006. doi: 10.15252/embj.2019104006. Epub 2020 Jun , 22. PMID:32567727<ref>PMID:32567727</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7bti" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Actin 3D structures|Actin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Gallus gallus]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Kumari, A]] | [[Category: Kumari, A]] | ||
| - | [[Category: Ragunath, V | + | [[Category: Ragunath, V K]] |
[[Category: Sirajuddin, M]] | [[Category: Sirajuddin, M]] | ||
| + | [[Category: Adp-f-actin]] | ||
| + | [[Category: Contractile protein]] | ||
| + | [[Category: Contractile protein-protein binding complex]] | ||
| + | [[Category: F-actin]] | ||
Current revision
Phalloidin bound F-actin complex
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