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| | <StructureSection load='2i0k' size='340' side='right'caption='[[2i0k]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='2i0k' size='340' side='right'caption='[[2i0k]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2i0k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/'brevibacterium_sterolicum' 'brevibacterium sterolicum']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I0K OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2I0K FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2i0k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacterium_sterolicum Brevibacterium sterolicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I0K FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1i19|1i19]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i0k OCA], [https://pdbe.org/2i0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i0k RCSB], [https://www.ebi.ac.uk/pdbsum/2i0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i0k ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2i0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i0k OCA], [http://pdbe.org/2i0k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2i0k RCSB], [http://www.ebi.ac.uk/pdbsum/2i0k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2i0k ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7SID9_BREST Q7SID9_BREST] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Brevibacterium sterolicum]] | | [[Category: Brevibacterium sterolicum]] |
| - | [[Category: Cholesterol oxidase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lim, L]] | + | [[Category: Lim L]] |
| - | [[Category: Vrielink, A]] | + | [[Category: Vrielink A]] |
| - | [[Category: Covalent fad]]
| + | |
| - | [[Category: Flavoenzyme]]
| + | |
| - | [[Category: Mix alpha beta]]
| + | |
| - | [[Category: Oxidoreductase]]
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| Structural highlights
Function
Q7SID9_BREST
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Cholesterol oxidase is a monomeric flavoenzyme that catalyses the oxidation of cholesterol to cholest-5-en-3-one followed by isomerization to cholest-4-en-3-one. The enzyme from Brevibacterium sterolicum contains the FAD cofactor covalently bound to His121. It was previously demonstrated that the H121A substitution results in a approximately 100 mV decrease in the midpoint redox potential and a approximately 40-fold decrease in turnover number compared to wild-type enzyme [Motteran, Pilone, Molla, Ghisla and Pollegioni (2001) Journal of Biological Chemistry 276, 18024-18030]. A detailed kinetic analysis of the H121A mutant enzyme shows that the decrease in turnover number is largely due to a corresponding decrease in the rate constant of flavin reduction, whilst the re-oxidation reaction is only marginally altered and the isomerization reaction is not affected by the substitution and precedes product dissociation. The X-ray structure of the mutant protein, determined to 1.7 A resolution (1 A identical with 0.1 nm), reveals only minor changes in the overall fold of the protein, namely: two loops have slight movements and a tryptophan residue changes conformation by a rotation of 180 degrees about chi1 compared to the native enzyme. Comparison of the isoalloxazine ring moiety of the FAD cofactor between the structures of the native and mutant proteins shows a change from a non-planar to a planar geometry (resulting in a more tetrahedral-like geometry for N5). This change is proposed to be a major factor contributing to the observed alteration in redox potential. Since a similar distortion of the flavin has not been observed in other covalent flavoproteins, it is proposed to represent a specific mode to facilitate flavin reduction in covalent cholesterol oxidase.
Structural and kinetic analyses of the H121A mutant of cholesterol oxidase.,Lim L, Molla G, Guinn N, Ghisla S, Pollegioni L, Vrielink A Biochem J. 2006 Nov 15;400(1):13-22. PMID:16856877[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lim L, Molla G, Guinn N, Ghisla S, Pollegioni L, Vrielink A. Structural and kinetic analyses of the H121A mutant of cholesterol oxidase. Biochem J. 2006 Nov 15;400(1):13-22. PMID:16856877 doi:10.1042/BJ20060664
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