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| | <StructureSection load='5djw' size='340' side='right'caption='[[5djw]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='5djw' size='340' side='right'caption='[[5djw]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5djw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bactn Bactn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DJW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5djw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DJW FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f7c|5f7c]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BT_3299 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226186 BACTN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5djw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djw OCA], [https://pdbe.org/5djw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5djw RCSB], [https://www.ebi.ac.uk/pdbsum/5djw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5djw ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5djw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5djw OCA], [http://pdbe.org/5djw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5djw RCSB], [http://www.ebi.ac.uk/pdbsum/5djw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5djw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q8A2K6_BACTN Q8A2K6_BACTN] |
| - | The human digestive system is host to a highly populated ecosystem of bacterial species that significantly contributes to our assimilation of dietary carbohydrates. Bacteroides thetaiotaomicron is a member of this ecosystem, and participates largely in the role of the gut microbiome by breaking down dietary complex carbohydrates. This process of acquiring glycans from the colon lumen is predicted to rely on the mechanisms of proteins that are part of a classified system known as polysaccharide utilization loci (PUL). These loci are responsible for binding substrates at the cell outer membrane, internalizing them, and then hydrolyzing them within the periplasm into simple sugars. Here we report our investigation into specific components of a PUL, and suggest an alternative starch utilization system in B. thetaiotaomicron. Our analysis of an outer membrane binding protein, a SusD homolog, highlights its contribution to this PUL by acquiring starch-based sugars from the colon lumen. Through our structural characterization of two Family GH31 alpha-glucosidases, we reveal the flexibility of this bacterium with respect to utilizing a range of starch-derived glycans with an emphasis on branched substrates. With these results we demonstrate the predicted function of a gene locus that is capable of contributing to starch hydrolysis in the human colon.
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| - | Suggested alternative starch utilization system from the human gut bacterium Bacteroides thetaiotaomicron.,Chaudet MM, Rose DR Biochem Cell Biol. 2016 Feb 10:1-6. PMID:27093479<ref>PMID:27093479</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 5djw" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]] | | *[[Alpha-glucosidase 3D structures|Alpha-glucosidase 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bactn]] | + | [[Category: Bacteroides thetaiotaomicron VPI-5482]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chaudet, M M]] | + | [[Category: Chaudet MM]] |
| - | [[Category: Rose, D R]] | + | [[Category: Rose DR]] |
| - | [[Category: Alpha-glucosidase]]
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| - | [[Category: Carbohydrate binding protein]]
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| - | [[Category: Family glycoside hydrolase 31]]
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