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| | <SX load='1gw7' size='340' side='right' viewer='molstar' caption='[[1gw7]], [[Resolution|resolution]] 13.50Å' scene=''> | | <SX load='1gw7' size='340' side='right' viewer='molstar' caption='[[1gw7]], [[Resolution|resolution]] 13.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1gw7]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpprd Bpprd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GW7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1GW7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1gw7]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_PRD1 Enterobacteria phage PRD1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GW7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GW7 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1cjd|1cjd]], [[1hb5|1hb5]], [[1hb7|1hb7]], [[1hqn|1hqn]], [[1hx6|1hx6]], [[1gw8|1gw8]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1gw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gw7 OCA], [http://pdbe.org/1gw7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gw7 RCSB], [http://www.ebi.ac.uk/pdbsum/1gw7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gw7 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gw7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gw7 OCA], [https://pdbe.org/1gw7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gw7 RCSB], [https://www.ebi.ac.uk/pdbsum/1gw7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gw7 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CAPSD_BPPRD CAPSD_BPPRD]] Major capsid protein self-assembles to form an icosahedral capsid with a pseudo T=25 symmetry, about 66 nm in diameter, and consisting of 240 capsid proteins trimers. The capsid encapsulates an inner membrane and the genomic dsDNA genome. The major coat protein P3 and two assembly factors (P10 and P17) are needed during the assembly of the virus particle inside the host cell, when the capsid protein multimers are capable of enclosing the host-derived membrane, containing the virus-encoded membrane-associated proteins. | + | [https://www.uniprot.org/uniprot/CAPSD_BPPRD CAPSD_BPPRD] Major capsid protein self-assembles to form an icosahedral capsid with a pseudo T=25 symmetry, about 66 nm in diameter, and consisting of 240 capsid proteins trimers. The capsid encapsulates an inner membrane and the genomic dsDNA genome. The major coat protein P3 and two assembly factors (P10 and P17) are needed during the assembly of the virus particle inside the host cell, when the capsid protein multimers are capable of enclosing the host-derived membrane, containing the virus-encoded membrane-associated proteins. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Bpprd]] | + | [[Category: Enterobacteria phage PRD1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bamford, D H]] | + | [[Category: Bamford DH]] |
| - | [[Category: Bamford, J K.H]] | + | [[Category: Bamford JKH]] |
| - | [[Category: Burnett, R M]] | + | [[Category: Burnett RM]] |
| - | [[Category: Butcher, S J]] | + | [[Category: Butcher SJ]] |
| - | [[Category: Fuller, S D]] | + | [[Category: Fuller SD]] |
| - | [[Category: Huiskonen, J]] | + | [[Category: Huiskonen J]] |
| - | [[Category: Martin, C San]] | + | [[Category: San Martin C]] |
| - | [[Category: Bacteriophage prd1]]
| + | |
| - | [[Category: Cryo- em]]
| + | |
| - | [[Category: Icosahedral virus]]
| + | |
| - | [[Category: Image reconstruction]]
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| - | [[Category: Tectiviridae]]
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| - | [[Category: Virus-viral protein complex]]
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| - | [[Category: Virus/viral protein]]
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| Structural highlights
Function
CAPSD_BPPRD Major capsid protein self-assembles to form an icosahedral capsid with a pseudo T=25 symmetry, about 66 nm in diameter, and consisting of 240 capsid proteins trimers. The capsid encapsulates an inner membrane and the genomic dsDNA genome. The major coat protein P3 and two assembly factors (P10 and P17) are needed during the assembly of the virus particle inside the host cell, when the capsid protein multimers are capable of enclosing the host-derived membrane, containing the virus-encoded membrane-associated proteins.
Publication Abstract from PubMed
Bacteriophage PRD1 shares many structural and functional similarities with adenovirus. A major difference is the PRD1 internal membrane, which acts in concert with vertex proteins to translocate the phage genome into the host. Multiresolution models of the PRD1 capsid, together with genetic analyses, provide fine details of the molecular interactions associated with particle stability and membrane dynamics. The N- and C-termini of the major coat protein (P3), which are required for capsid assembly, act as conformational switches bridging capsid to membrane and linking P3 trimers. Electrostatic P3-membrane interactions increase virion stability upon DNA packaging. Newly revealed proteins suggest how the metastable vertex works and how the capsid edges are stabilized.
Minor proteins, mobile arms and membrane-capsid interactions in the bacteriophage PRD1 capsid.,San Martin C, Huiskonen JT, Bamford JK, Butcher SJ, Fuller SD, Bamford DH, Burnett RM Nat Struct Biol. 2002 Oct;9(10):756-63. PMID:12219080[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ San Martin C, Huiskonen JT, Bamford JK, Butcher SJ, Fuller SD, Bamford DH, Burnett RM. Minor proteins, mobile arms and membrane-capsid interactions in the bacteriophage PRD1 capsid. Nat Struct Biol. 2002 Oct;9(10):756-63. PMID:12219080 doi:10.1038/nsb837
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