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| | <SX load='2xvr' size='340' side='right' viewer='molstar' caption='[[2xvr]], [[Resolution|resolution]] 10.80Å' scene=''> | | <SX load='2xvr' size='340' side='right' viewer='molstar' caption='[[2xvr]], [[Resolution|resolution]] 10.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2xvr]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XVR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2XVR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xvr]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XVR FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2xvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xvr OCA], [http://pdbe.org/2xvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2xvr RCSB], [http://www.ebi.ac.uk/pdbsum/2xvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2xvr ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10.8Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xvr OCA], [https://pdbe.org/2xvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xvr RCSB], [https://www.ebi.ac.uk/pdbsum/2xvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xvr ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CAPSA_BPT7 CAPSA_BPT7] Assembles with the minor capsid protein to form an icosahedral capsid with a T=7 symmetry, about 60 nm in diameter, and consisting of 415 capsid proteins. The major and minor capsid proteins are incorporated into the capsid in about a 90/10 ratio respectively. Once the capsid is formed, encapsidates one single copy of the viral genome.<ref>PMID:20962334</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Enterobacteria phage t7]] | + | [[Category: Escherichia phage T7]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Carrascosa, J L]] | + | [[Category: Carrascosa JL]] |
| - | [[Category: Caston, J R]] | + | [[Category: Caston JR]] |
| - | [[Category: Cuervo, A]] | + | [[Category: Cuervo A]] |
| - | [[Category: Ionel, A]] | + | [[Category: Ionel A]] |
| - | [[Category: Luque, D]] | + | [[Category: Luque D]] |
| - | [[Category: Martin-Benito, J]] | + | [[Category: Martin-Benito J]] |
| - | [[Category: Valpuesta, J M]] | + | [[Category: Valpuesta JM]] |
| - | [[Category: Velazquez-Muriel, J A]] | + | [[Category: Velazquez-Muriel JA]] |
| - | [[Category: Capsid maturation]]
| + | |
| - | [[Category: Morphogenetic intermediate]]
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| - | [[Category: Virus]]
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| Structural highlights
Function
CAPSA_BPT7 Assembles with the minor capsid protein to form an icosahedral capsid with a T=7 symmetry, about 60 nm in diameter, and consisting of 415 capsid proteins. The major and minor capsid proteins are incorporated into the capsid in about a 90/10 ratio respectively. Once the capsid is formed, encapsidates one single copy of the viral genome.[1]
Publication Abstract from PubMed
Maturation of dsDNA bacteriophages involves assembling the virus prohead from a limited set of structural components, followed by rearrangements required for the stability that is necessary for infecting a host under challenging environmental conditions. Here, we determine the mature capsid structure of T7 at 1 nm resolution by cryo electron microscopy, and compare it with the prohead to reveal the molecular basis of T7 shell maturation. The mature capsid presents an expanded and thinner shell, with a drastic rearrangement of the major protein monomers that increases in their interacting surfaces, in turn resulting in a new bonding lattice. The rearrangements include tilting, in-plane rotation and radial expansion of the subunits, as well as a relative bending of the A and P-domains of each subunit. The unique features of this shell transformation, which does not employ the accessory proteins, inserted domains, or molecular interactions observed in other phages, suggest a simple capsid assembling strategy that may have appeared early in the evolution of these viruses.
Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7.,Ionel A, Velazquez-Muriel JA, Luque D, Cuervo A, Caston JR, Valpuesta JM, Martin-Benito J, Carrascosa JL J Biol Chem. 2010 Oct 20. PMID:20962334[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ionel A, Velazquez-Muriel JA, Luque D, Cuervo A, Caston JR, Valpuesta JM, Martin-Benito J, Carrascosa JL. Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7. J Biol Chem. 2010 Oct 20. PMID:20962334 doi:10.1074/jbc.M110.187211
- ↑ Ionel A, Velazquez-Muriel JA, Luque D, Cuervo A, Caston JR, Valpuesta JM, Martin-Benito J, Carrascosa JL. Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7. J Biol Chem. 2010 Oct 20. PMID:20962334 doi:10.1074/jbc.M110.187211
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