2ypw

From Proteopedia

(Difference between revisions)

Current revision

Atomic model for the N-terminus of TraO fitted in the full-length structure of the bacterial pKM101 type IV secretion system core complex

2ypw, resolution 12.40Å

Structural highlights

2ypw is a 14 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 12.4Å
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q46704_ECOLX

Publication Abstract from PubMed

Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4. VirB7, 9 and 10 assemble into a 1.07 MegaDalton membrane-spanning core complex (CC), around which all other components assemble. This complex is made of two parts, the O-layer inserted in the outer membrane and the I-layer inserted in the inner membrane. While the structure of the O-layer has been solved by X-ray crystallography, there is no detailed structural information on the I-layer. Using high-resolution cryo-electron microscopy and molecular modelling combined with biochemical approaches, we determined the I-layer structure and located its various components in the electron density. Our results provide new structural insights on the CC, from which the essential features of T4S system mechanisms can be derived.

Structure of a bacterial type IV secretion core complex at subnanometre resolution.,Rivera-Calzada A, Fronzes R, Savva CG, Chandran V, Lian PW, Laeremans T, Pardon E, Steyaert J, Remaut H, Waksman G, Orlova EV EMBO J. 2013 Mar 19. doi: 10.1038/emboj.2013.58. PMID:23511972^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rivera-Calzada A, Fronzes R, Savva CG, Chandran V, Lian PW, Laeremans T, Pardon E, Steyaert J, Remaut H, Waksman G, Orlova EV. Structure of a bacterial type IV secretion core complex at subnanometre resolution. EMBO J. 2013 Mar 19. doi: 10.1038/emboj.2013.58. PMID:23511972 doi:http://dx.doi.org/10.1038/emboj.2013.58

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ypw"

@@ Line 3: / Line 3: @@
 <SX load='2ypw' size='340' side='right' viewer='molstar' caption='[[2ypw]], [[Resolution|resolution]] 12.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2ypw]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YPW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2YPW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2ypw]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YPW FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2ypw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ypw OCA], [http://pdbe.org/2ypw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ypw RCSB], [http://www.ebi.ac.uk/pdbsum/2ypw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ypw ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12.4&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ypw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ypw OCA], [https://pdbe.org/2ypw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ypw RCSB], [https://www.ebi.ac.uk/pdbsum/2ypw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ypw ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q46704_ECOLX Q46704_ECOLX]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 22: @@
 __TOC__
 </SX>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Chandran, V]]
+[[Category: Chandran V]]
-[[Category: Fronzes, R]]
+[[Category: Fronzes R]]
-[[Category: Laeremans, T]]
+[[Category: Laeremans T]]
-[[Category: Lian, P W]]
+[[Category: Lian PW]]
-[[Category: Orlova, E V]]
+[[Category: Orlova EV]]
-[[Category: Pardon, E]]
+[[Category: Pardon E]]
-[[Category: Remaut, H]]
+[[Category: Remaut H]]
-[[Category: Rivera-Calzada, A]]
+[[Category: Rivera-Calzada A]]
-[[Category: Savva, C G]]
+[[Category: Savva CG]]
-[[Category: Steyaert, J]]
+[[Category: Steyaert J]]
-[[Category: Waksman, G]]
+[[Category: Waksman G]]
-[[Category: Bacterial secretion]]
-[[Category: Membrane protein]]
-[[Category: Type iv secretion]]

2ypw

From Proteopedia

Current revision

Atomic model for the N-terminus of TraO fitted in the full-length structure of the bacterial pKM101 type IV secretion system core complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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