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1s5x

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(New page: 200px<br /> <applet load="1s5x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s5x, resolution 2.4&Aring;" /> '''The crystal structur...)
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[[Image:1s5x.gif|left|200px]]<br />
 
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<applet load="1s5x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s5x, resolution 2.4&Aring;" />
 
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'''The crystal structure of Trematomus bernacchii hemoglobin oxidized by air'''<br />
 
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==Overview==
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==The crystal structure of Trematomus bernacchii hemoglobin oxidized by air==
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Analysis of the molecular properties of proteins extracted from organisms, living under extreme conditions often highlights peculiar features. We, investigated by UV-visible spectroscopy and X-ray crystallography the, oxidation process, promoted by air or ferricyanide, of five hemoglobins, extracted from Antarctic fishes (Notothenioidei). Spectroscopic analysis, revealed that these hemoglobins share a common oxidation pathway, which, shows striking differences from the oxidation processes of hemoglobins, from other vertebrates. Indeed, simple exposure of these hemoglobins to, air leads to the formation of a significant amount of the low-spin, hexacoordinated form, denoted hemichrome. This hemichrome form, which is, detected under a variety of experimental conditions, can be reversibly, transformed to either carbomonoxy or deoxygenated forms with reducing, agents. Interestingly, the spectra of the fully oxidized species, obtained, by treating the protein with ferricyanide, show the simultaneous presence, of peaks corresponding to different hexacoordinated states, the aquomet, and the hemichrome. In order to assign the heme region state of the alpha, and beta chains, the air-oxidized and ferricyanide-oxidized forms of, Trematomus bernacchii hemoglobin were crystallized. Crystallographic, analysis revealed that these forms correspond to an, alpha(aquomet)-beta(bishistidyl-hemichrome) state. This demonstrates that, the alpha and beta chains of Antarctic fish hemoglobins follow very, different oxidation pathways. As found for Trematomus newnesi hemoglobin, in a partial hemichrome state [Riccio, A., Vitagliano, L., di Prisco, G., Zagari, A. &amp; Mazzarella, L. (2002) Proc. Natl Acad. Sci. USA99, 9801-9806], the quaternary structures of these, alpha(aquomet)-beta(bishistidyl-hemichrome) forms are intermediate between, the physiological R and T hemoglobin states. Together, these structures, provide information on the general features of this intermediate state.
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<StructureSection load='1s5x' size='340' side='right'caption='[[1s5x]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1s5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trematomus_bernacchii Trematomus bernacchii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S5X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5x OCA], [https://pdbe.org/1s5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s5x RCSB], [https://www.ebi.ac.uk/pdbsum/1s5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s5x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HBA_TREBE HBA_TREBE] Involved in oxygen transport from gills to the various peripheral tissues.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s5/1s5x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s5x ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1S5X is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Trematomus_bernacchii Trematomus bernacchii] with ACE and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S5X OCA].
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The oxidation process of Antarctic fish hemoglobins., Vitagliano L, Bonomi G, Riccio A, di Prisco G, Smulevich G, Mazzarella L, Eur J Biochem. 2004 May;271(9):1651-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15096204 15096204]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Trematomus bernacchii]]
[[Category: Trematomus bernacchii]]
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[[Category: Bonomi, G.]]
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[[Category: Bonomi G]]
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[[Category: Mazzarella, L.]]
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[[Category: Mazzarella L]]
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[[Category: Prisco, G.di.]]
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[[Category: Riccio A]]
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[[Category: Riccio, A.]]
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[[Category: Smulevich G]]
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[[Category: Smulevich, G.]]
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[[Category: Vitagliano L]]
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[[Category: Vitagliano, L.]]
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[[Category: Di Prisco G]]
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[[Category: ACE]]
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[[Category: HEM]]
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[[Category: bis-histidine]]
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[[Category: hemichrome]]
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[[Category: oxidation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 13:17:38 2007''
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Current revision

The crystal structure of Trematomus bernacchii hemoglobin oxidized by air

PDB ID 1s5x

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