3izg

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<SX load='3izg' size='340' side='right' viewer='molstar' caption='[[3izg]], [[Resolution|resolution]] 10.90&Aring;' scene=''>
<SX load='3izg' size='340' side='right' viewer='molstar' caption='[[3izg]], [[Resolution|resolution]] 10.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3izg]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IZG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3IZG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3izg]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IZG FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3izg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3izg OCA], [http://pdbe.org/3izg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3izg RCSB], [http://www.ebi.ac.uk/pdbsum/3izg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3izg ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3izg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3izg OCA], [https://pdbe.org/3izg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3izg RCSB], [https://www.ebi.ac.uk/pdbsum/3izg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3izg ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/CAPSA_BPT7 CAPSA_BPT7] Assembles with the minor capsid protein to form an icosahedral capsid with a T=7 symmetry, about 60 nm in diameter, and consisting of 415 capsid proteins. The major and minor capsid proteins are incorporated into the capsid in about a 90/10 ratio respectively. Once the capsid is formed, encapsidates one single copy of the viral genome.<ref>PMID:20962334</ref>
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Maturation of dsDNA bacteriophages involves assembling the virus prohead from a limited set of structural components, followed by rearrangements required for the stability that is necessary for infecting a host under challenging environmental conditions. Here, we determine the mature capsid structure of T7 at 1 nm resolution by cryo electron microscopy, and compare it with the prohead to reveal the molecular basis of T7 shell maturation. The mature capsid presents an expanded and thinner shell, with a drastic rearrangement of the major protein monomers that increases in their interacting surfaces, in turn resulting in a new bonding lattice. The rearrangements include tilting, in-plane rotation and radial expansion of the subunits, as well as a relative bending of the A and P-domains of each subunit. The unique features of this shell transformation, which does not employ the accessory proteins, inserted domains, or molecular interactions observed in other phages, suggest a simple capsid assembling strategy that may have appeared early in the evolution of these viruses.
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Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7.,Ionel A, Velazquez-Muriel JA, Luque D, Cuervo A, Caston JR, Valpuesta JM, Martin-Benito J, Carrascosa JL J Biol Chem. 2010 Oct 20. PMID:20962334<ref>PMID:20962334</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3izg" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</SX>
</SX>
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[[Category: Enterobacteria phage t7]]
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[[Category: Escherichia phage T7]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Agirrezabala, X]]
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[[Category: Agirrezabala X]]
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[[Category: Carrascosa, J L]]
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[[Category: Carrascosa JL]]
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[[Category: Caston, J R]]
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[[Category: Caston JR]]
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[[Category: Cuervo, A]]
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[[Category: Cuervo A]]
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[[Category: Ionel, A]]
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[[Category: Ionel A]]
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[[Category: Luque, D]]
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[[Category: Luque D]]
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[[Category: Martin-Benito, J]]
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[[Category: Martin-Benito J]]
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[[Category: Valpuesta, J M]]
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[[Category: Valpuesta JM]]
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[[Category: Velazquez-Muriel, J A]]
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[[Category: Velazquez-Muriel JA]]
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[[Category: Bacteriophage]]
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[[Category: Capsid maturation]]
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[[Category: Cryoelectron microscopy]]
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[[Category: Icosahedral]]
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[[Category: Morphogenetic intermediate]]
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[[Category: Virus]]
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Current revision

Bacteriophage T7 prohead shell EM-derived atomic model

3izg, resolution 10.90Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

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