3j1z

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<SX load='3j1z' size='340' side='right' viewer='molstar' caption='[[3j1z]], [[Resolution|resolution]] 13.00&Aring;' scene=''>
<SX load='3j1z' size='340' side='right' viewer='molstar' caption='[[3j1z]], [[Resolution|resolution]] 13.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3j1z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sheon Sheon]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J1Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J1Z FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3j1z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_oneidensis_MR-1 Shewanella oneidensis MR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J1Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J1Z FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SO_4475 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=211586 SHEON])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 13&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1z OCA], [http://pdbe.org/3j1z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j1z RCSB], [http://www.ebi.ac.uk/pdbsum/3j1z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j1z ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1z OCA], [https://pdbe.org/3j1z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j1z RCSB], [https://www.ebi.ac.uk/pdbsum/3j1z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j1z ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q8E919_SHEON Q8E919_SHEON]
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YiiP is a dimeric Zn(2+)/H(+) antiporter from Escherichia coli belonging to the cation diffusion facilitator family. We used cryoelectron microscopy to determine a 13-A resolution structure of a YiiP homolog from Shewanella oneidensis within a lipid bilayer in the absence of Zn(2+). Starting from the X-ray structure in the presence of Zn(2+), we used molecular dynamics flexible fitting to build a model consistent with our map. Comparison of the structures suggests a conformational change that involves pivoting of a transmembrane, four-helix bundle (M1, M2, M4, and M5) relative to the M3-M6 helix pair. Although accessibility of transport sites in the X-ray model indicates that it represents an outward-facing state, our model is consistent with an inward-facing state, suggesting that the conformational change is relevant to the alternating access mechanism for transport. Molecular dynamics simulation of YiiP in a lipid environment was used to address the feasibility of this conformational change. Association of the C-terminal domains is the same in both states, and we speculate that this association is responsible for stabilizing the dimer that, in turn, may coordinate the rearrangement of the transmembrane helices.
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Inward-facing conformation of the zinc transporter YiiP revealed by cryoelectron microscopy.,Coudray N, Valvo S, Hu M, Lasala R, Kim C, Vink M, Zhou M, Provasi D, Filizola M, Tao J, Fang J, Penczek PA, Ubarretxena-Belandia I, Stokes DL Proc Natl Acad Sci U S A. 2013 Jan 22. PMID:23341604<ref>PMID:23341604</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3j1z" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</SX>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Sheon]]
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[[Category: Shewanella oneidensis MR-1]]
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[[Category: Coudray, N]]
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[[Category: Coudray N]]
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[[Category: Fang, J]]
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[[Category: Fang J]]
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[[Category: Filizola, M]]
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[[Category: Filizola M]]
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[[Category: Hu, M]]
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[[Category: Hu M]]
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[[Category: Kim, C]]
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[[Category: Kim C]]
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[[Category: Lasala, R]]
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[[Category: Lasala R]]
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[[Category: Penczek, P A]]
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[[Category: Penczek PA]]
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[[Category: Provasi, D]]
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[[Category: Provasi D]]
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[[Category: Stokes, D L]]
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[[Category: Stokes DL]]
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[[Category: TEMIMPS, Transcontinental EM Initiative for Membrane Protein Structure]]
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[[Category: Tao J]]
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[[Category: Tao, J]]
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[[Category: Ubarretxena-Belandia I]]
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[[Category: Ubarretxena-Belandia, I]]
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[[Category: Valvo S]]
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[[Category: Valvo, S]]
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[[Category: Vink M]]
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[[Category: Vink, M]]
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[[Category: Zhou M]]
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[[Category: Zhou, M]]
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[[Category: Alternating access mechanism]]
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[[Category: Metal transport]]
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[[Category: Psi-biology]]
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[[Category: Secondary transporter]]
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[[Category: Structural genomic]]
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[[Category: Temimp]]
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[[Category: Transcontinental em initiative for membrane protein structure]]
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[[Category: Zinc transporter]]
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Current revision

Inward-Facing Conformation of the Zinc Transporter YiiP revealed by Cryo-electron Microscopy

3j1z, resolution 13.00Å

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