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3j6u

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<SX load='3j6u' size='340' side='right' viewer='molstar' caption='[[3j6u]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
<SX load='3j6u' size='340' side='right' viewer='molstar' caption='[[3j6u]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J6U FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3j6u]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_3 Dengue virus 3] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J6U FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j6s|3j6s]], [[3j6t|3j6t]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [http://pdbe.org/3j6u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [http://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6u OCA], [https://pdbe.org/3j6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j6u RCSB], [https://www.ebi.ac.uk/pdbsum/3j6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6u ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/Q6DLV0_9FLAV Q6DLV0_9FLAV]] Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).[SAAS:SAAS000336_004_099774]
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[https://www.uniprot.org/uniprot/A9LID6_9FLAV A9LID6_9FLAV]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Dengue virus (DENV) infects ~400 million people annually. There is no licensed vaccine or therapeutic drug. Only a small fraction of the total DENV-specific antibodies in a naturally occurring dengue infection consists of highly neutralizing antibodies. Here we show that the DENV-specific human monoclonal antibody 5J7 is exceptionally potent, neutralizing 50% of virus at nanogram-range antibody concentration. The 9 A resolution cryo-electron microscopy structure of the Fab 5J7-DENV complex shows that a single Fab molecule binds across three envelope proteins and engages three functionally important domains, each from a different envelope protein. These domains are critical for receptor binding and fusion to the endosomal membrane. The ability to bind to multiple domains allows the antibody to fully coat the virus surface with only 60 copies of Fab, that is, half the amount compared with other potent antibodies. Our study reveals a highly efficient and unusual mechanism of molecular recognition by an antibody.
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A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins.,Fibriansah G, Tan JL, Smith SA, de Alwis R, Ng TS, Kostyuchenko VA, Jadi RS, Kukkaro P, de Silva AM, Crowe JE, Lok SM Nat Commun. 2015 Feb 20;6:6341. doi: 10.1038/ncomms7341. PMID:25698059<ref>PMID:25698059</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3j6u" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</SX>
</SX>
[[Category: Dengue virus 3]]
[[Category: Dengue virus 3]]
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[[Category: Human]]
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Alwis, R de]]
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[[Category: Crowe Jr JE]]
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[[Category: Crowe, J E]]
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[[Category: Fibriansah G]]
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[[Category: Fibriansah, G]]
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[[Category: Kostyuchenko VA]]
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[[Category: Kostyuchenko, V A]]
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[[Category: Kukkaro P]]
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[[Category: Kukkaro, P]]
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[[Category: Lok S-M]]
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[[Category: Lok, S M]]
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[[Category: Ng T-S]]
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[[Category: Ng, T S]]
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[[Category: Smith SA]]
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[[Category: Silva, A M.de]]
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[[Category: Tan JL]]
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[[Category: Smith, S A]]
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[[Category: De Alwis R]]
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[[Category: Tan, J L]]
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[[Category: De Silva AM]]
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[[Category: Dengue virus]]
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[[Category: Human antibody]]
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[[Category: Neutralization]]
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[[Category: Virus-immune system complex]]
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Current revision

Cryo-EM structure of Dengue virus serotype 3 in complex with human antibody 5J7 Fab

3j6u, resolution 9.00Å

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