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3j7v

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<SX load='3j7v' size='340' side='right' viewer='molstar' caption='[[3j7v]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
<SX load='3j7v' size='340' side='right' viewer='molstar' caption='[[3j7v]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3j7v]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3J7V FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3j7v]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J7V FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j7w|3j7w]], [[3j7x|3j7x]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3j7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7v OCA], [http://pdbe.org/3j7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j7v RCSB], [http://www.ebi.ac.uk/pdbsum/3j7v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7v ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7v OCA], [https://pdbe.org/3j7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j7v RCSB], [https://www.ebi.ac.uk/pdbsum/3j7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j7v ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/CAPSA_BPT7 CAPSA_BPT7] Assembles with the minor capsid protein to form an icosahedral capsid with a T=7 symmetry, about 60 nm in diameter, and consisting of 415 capsid proteins. The major and minor capsid proteins are incorporated into the capsid in about a 90/10 ratio respectively. Once the capsid is formed, encapsidates one single copy of the viral genome.<ref>PMID:20962334</ref>
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Many dsDNA viruses first assemble a DNA-free procapsid, using a scaffolding protein-dependent process. The procapsid, then, undergoes dramatic conformational maturation while packaging DNA. For bacteriophage T7 we report the following four single-particle cryo-EM 3D reconstructions and the derived atomic models: procapsid (4.6-A resolution), an early-stage DNA packaging intermediate (3.5 A), a later-stage packaging intermediate (6.6 A), and the final infectious phage (3.6 A). In the procapsid, the N terminus of the major capsid protein, gp10, has a six-turn helix at the inner surface of the shell, where each skewed hexamer of gp10 interacts with two scaffolding proteins. With the exit of scaffolding proteins during maturation the gp10 N-terminal helix unfolds and swings through the capsid shell to the outer surface. The refolded N-terminal region has a hairpin that forms a novel noncovalent, joint-like, intercapsomeric interaction with a pocket formed during shell expansion. These large conformational changes also result in a new noncovalent, intracapsomeric topological linking. Both interactions further stabilize the capsids by interlocking all pentameric and hexameric capsomeres in both DNA packaging intermediate and phage. Although the final phage shell has nearly identical structure to the shell of the DNA-free intermediate, surprisingly we found that the icosahedral faces of the phage are slightly ( approximately 4 A) contracted relative to the faces of the intermediate, despite the internal pressure from the densely packaged DNA genome. These structures provide a basis for understanding the capsid maturation process during DNA packaging that is essential for large numbers of dsDNA viruses.
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Capsid expansion mechanism of bacteriophage T7 revealed by multistate atomic models derived from cryo-EM reconstructions.,Guo F, Liu Z, Fang PA, Zhang Q, Wright ET, Wu W, Zhang C, Vago F, Ren Y, Jakana J, Chiu W, Serwer P, Jiang W Proc Natl Acad Sci U S A. 2014 Oct 13. pii: 201407020. PMID:25313071<ref>PMID:25313071</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3j7v" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</SX>
</SX>
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[[Category: Enterobacteria phage t7]]
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[[Category: Escherichia phage T7]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Chiu, W]]
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[[Category: Chiu W]]
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[[Category: Fang, P A]]
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[[Category: Fang PA]]
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[[Category: Guo, F]]
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[[Category: Guo F]]
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[[Category: Jakata, J]]
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[[Category: Jakata J]]
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[[Category: Jiang, W]]
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[[Category: Jiang W]]
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[[Category: Liu, Z]]
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[[Category: Liu Z]]
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[[Category: Ren, Y]]
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[[Category: Ren Y]]
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[[Category: Serwer, P]]
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[[Category: Serwer P]]
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[[Category: Vago, F]]
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[[Category: Vago F]]
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[[Category: Wright, E T]]
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[[Category: Wright ET]]
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[[Category: Wu, W]]
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[[Category: Wu W]]
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[[Category: Zhang, C]]
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[[Category: Zhang C]]
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[[Category: Zhang, Q]]
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[[Category: Zhang Q]]
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[[Category: Dna packaging]]
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[[Category: Maturation]]
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[[Category: Non-covalent topological linking]]
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[[Category: Procapsid]]
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[[Category: Virus]]
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Current revision

Capsid Expansion Mechanism Of Bacteriophage T7 Revealed By Multi-State Atomic Models Derived From Cryo-EM Reconstructions

3j7v, resolution 4.50Å

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