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| | <SX load='4ard' size='340' side='right' viewer='molstar' caption='[[4ard]], [[Resolution|resolution]] 7.00Å' scene=''> | | <SX load='4ard' size='340' side='right' viewer='molstar' caption='[[4ard]], [[Resolution|resolution]] 7.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ard]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mpmv Mpmv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4ARD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ard]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mason-Pfizer_monkey_virus Mason-Pfizer monkey virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARD FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bax|1bax]], [[1cl4|1cl4]], [[4arg|4arg]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4ard FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ard OCA], [http://pdbe.org/4ard PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ard RCSB], [http://www.ebi.ac.uk/pdbsum/4ard PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ard ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ard FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ard OCA], [https://pdbe.org/4ard PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ard RCSB], [https://www.ebi.ac.uk/pdbsum/4ard PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ard ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GAG_MPMV GAG_MPMV]] p10 is the matrix protein. P14 is the nucleocapsid protein. p27 is the capsid protein. | + | [https://www.uniprot.org/uniprot/GAG_MPMV GAG_MPMV] p10 is the matrix protein. P14 is the nucleocapsid protein. p27 is the capsid protein. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mpmv]] | + | [[Category: Mason-Pfizer monkey virus]] |
| - | [[Category: Bharat, T A.M]] | + | [[Category: Bharat TAM]] |
| - | [[Category: Briggs, J A.G]] | + | [[Category: Briggs JAG]] |
| - | [[Category: Davey, N E]] | + | [[Category: Davey NE]] |
| - | [[Category: Marco, A D]] | + | [[Category: Marco AD]] |
| - | [[Category: Riches, J D]] | + | [[Category: Riches JD]] |
| - | [[Category: Ruml, T]] | + | [[Category: Ruml T]] |
| - | [[Category: Rumlova, M]] | + | [[Category: Rumlova M]] |
| - | [[Category: Sachse, C]] | + | [[Category: Sachse C]] |
| - | [[Category: Ulbrich, P]] | + | [[Category: Ulbrich P]] |
| - | [[Category: Gag]]
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| - | [[Category: Retrovirus]]
| + | |
| - | [[Category: Viral protein]]
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| Structural highlights
Function
GAG_MPMV p10 is the matrix protein. P14 is the nucleocapsid protein. p27 is the capsid protein.
Publication Abstract from PubMed
The assembly of retroviruses such as HIV-1 is driven by oligomerization of their major structural protein, Gag. Gag is a multidomain polyprotein including three conserved folded domains: MA (matrix), CA (capsid) and NC (nucleocapsid). Assembly of an infectious virion proceeds in two stages. In the first stage, Gag oligomerization into a hexameric protein lattice leads to the formation of an incomplete, roughly spherical protein shell that buds through the plasma membrane of the infected cell to release an enveloped immature virus particle. In the second stage, cleavage of Gag by the viral protease leads to rearrangement of the particle interior, converting the non-infectious immature virus particle into a mature infectious virion. The immature Gag shell acts as the pivotal intermediate in assembly and is a potential target for anti-retroviral drugs both in inhibiting virus assembly and in disrupting virus maturation. However, detailed structural information on the immature Gag shell has not previously been available. For this reason it is unclear what protein conformations and interfaces mediate the interactions between domains and therefore the assembly of retrovirus particles, and what structural transitions are associated with retrovirus maturation. Here we solve the structure of the immature retroviral Gag shell from Mason-Pfizer monkey virus by combining cryo-electron microscopy and tomography. The 8-A resolution structure permits the derivation of a pseudo-atomic model of CA in the immature retrovirus, which defines the protein interfaces mediating retrovirus assembly. We show that transition of an immature retrovirus into its mature infectious form involves marked rotations and translations of CA domains, that the roles of the amino-terminal and carboxy-terminal domains of CA in assembling the immature and mature hexameric lattices are exchanged, and that the CA interactions that stabilize the immature and mature viruses are almost completely distinct.
Structure of the immature retroviral capsid at 8 A resolution by cryo-electron microscopy.,Bharat TA, Davey NE, Ulbrich P, Riches JD, de Marco A, Rumlova M, Sachse C, Ruml T, Briggs JA Nature. 2012 Jul 19;487(7407):385-9. PMID:22722831[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Bharat TA, Davey NE, Ulbrich P, Riches JD, de Marco A, Rumlova M, Sachse C, Ruml T, Briggs JA. Structure of the immature retroviral capsid at 8 A resolution by cryo-electron microscopy. Nature. 2012 Jul 19;487(7407):385-9. PMID:22722831 doi:10.1038/nature11169
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